Sacha inchi seeds are a potential source of natural oil rich in essential polyunsaturated fatty acids and have various nutraceutical and health benefits. This study developed an aqueous enzymatic extraction process for eco-friendly extracting sacha inchi seed oil. The activities of different enzymes (proteases and cellulase) in the extraction process were evaluated. Among the tested enzymes, papain exhibited the highest extraction activity. The papain-assisted extraction was subsequently optimized through response surface methodology to yield maximum oil level. A maximum oil yield of 28.45% was obtained under the following extraction conditions: enzyme loading, 4.46%; water-to-sample ratio, 4.45 mL/g; extraction time, 4.95 h; and temperature, 38.9 °C. Under optimal extraction conditions, papain could be repeatedly used for extraction. This study indicates that papain is a potential protease and that aqueous enzymatic extraction is an eco-friendly and efficient process for extracting beneficial oil from sacha inchi seeds.

Sacha inchi种子是富含必需多不饱和脂肪酸的天然油脂的潜在来源，并具有多种营养保健和健康益处。这项研究开发了一种水酶提取工艺，可用于生态友好地提取sa茶种子油。评估了提取过程中不同酶（蛋白酶和纤维素酶）的活性。在测试的酶中，木瓜蛋白酶具有最高的提取活性。随后通过响应面法对木瓜蛋白酶辅助的提取进行了优化，以产生最大的油含量。在以下提取条件下，最大油收率为28.45％：酶负载率为4.46％；水样比为4.45 mL / g; 提取时间4.95小时；和温度，38.9°C。在最佳提取条件下，木瓜蛋白酶可重复用于提取。