Mycobacterium avium subsp. Paratuberculosis (Map) is an important animal pathogen with a worldwide distribution that may contribute to Crohn's disease in humans. This study aimed to investigate the behavior of Map in Lighvan cheese with special reference to the strains of Map, inoculum load, and storage time. One laboratory and two native strains of Map were inoculated (10² and 10³ Map cell/mL) to cheese milk. The behavior of Map throughout the manufacture, ripening, and storage stage of Lighvan cheese tracked using propidium monoazide (PMA) qPCR and culture examination. According to the results, PMA-qPCR showed parallel outcomes in comparison with the culture assay. Besides, in all cheese batches Map was not affected during the ripening; however, throughout the storage period Map population decreased depending on the strain of Map. Trend analysis revealed no significant difference between the persistence of sheep and goat strains, but in all cheese batches, these native strains persisted significantly (p < 0.05) longer than the laboratory strain. In all treatments, the decreasing rate of Map found independent of the initial inoculum load. Nonetheless, in the cheese batches with higher initial inoculum, Map persisted two months longer.

鸟分枝杆菌亚种 副结核病（Map）是一种重要的动物病原体，分布在世界各地，可能导致人类克罗恩氏病。这项研究旨在调查Lighvan奶酪中Map的行为，并特别参考Map的菌株，接种量和储存时间。将一个实验室和两个本地Map菌株接种到干酪乳中（10 ²和10 ³ Map细胞/ mL）。地图的行为使用单叠氮化丙锭（PMA）qPCR和培养检查来跟踪Lighvan奶酪的整个制造，成熟和存储阶段。根据结果​​，PMA-qPCR与培养分析相比显示出平行的结果。此外，在所有奶酪批次中，成熟过程中的Map均不受影响；但是，在整个存储期间，Map种群根据Map的应变而减少。趋势分析显示，绵羊和山羊菌株的持久性之间没有显着差异，但是在所有奶酪批次中，这些天然菌株比实验室菌株的持久时间长（p <0.05）。在所有治疗中，Map的降低率发现与初始接种量无关。尽管如此，在初始接种量较高的奶酪批次中，Map的存在时间要长两个月。