BACKGROUND

Aluminum (Al) has been reported to induce testicular injury via oxidative stress. Ananas comosus stem extract is an inexpensive byproduct waste rich in bromelain which is a group of sulfur-containing enzymes known for its biological activities and medicinal applications. So, the current investigation aims to evaluate the efficacy of bromelain in counteracting oxidative injury and testicular dysfunction stimulated by aluminum in rats.

METHODS

Male adult Wistar rats were divided into four groups. The first group used as control, however, the second and third groups were received bromelain (250 mg/kg) and AlCl₃ (34 mg/Kg, 1/25 LD₅₀), and the fourth group supplemented with bromelain one hour before AlCl₃ intoxication, respectively. Bromelain was administered daily while AlCl₃ was given every other day by oral gavages for one month.

RESULTS

Al intoxicated animals revealed an elevation in lipid peroxidation (TBARS and H₂O₂) level and lactate dehydrogenase (LDH) activity. However, reduced glutathione (GSH) and protein contents, antioxidant enzymes (SOD, CAT, GPx, GR, GST), phosphatases (ALP, AcP) and aminotransferases (AST, ALT) activities were significantly reduced. Additionally, considerable amendments in hormonal levels (testosterone, luteinizing and follicle-stimulating hormone) and sperm characteristics were spotted. Further, histological variations in the testes section were detected and this supports the biochemical observations. Otherwise, rats supplemented with bromelain alone diminished TBARS and H₂O₂ and augmented mostly other parameters. Furthermore, supplementation with bromelain before Al intoxication in rats exhibited worthy betterment in oxidative stress markers, hormones, and sperm quality compared to Al treated group.

CONCLUSION

In conclusion, bromelain had a powerful protective role against Al-induced testicular dysfunction so, it represents a novel approach in metal toxicity processing.

中文翻译：

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来自 Ananas comosus 茎的菠萝蛋白酶减轻铝引起的大鼠氧化毒性和睾丸功能障碍。

背景

据报道，铝 (Al) 可通过氧化应激诱导睾丸损伤。Ananas comosus茎提取物是一种廉价的副产品废物，富含菠萝蛋白酶，菠萝蛋白酶是一组以其生物活性和医学应用而闻名的含硫酶。因此，目前的研究旨在评估菠萝蛋白酶在抵抗铝刺激的大鼠氧化损伤和睾丸功能障碍中的功效。

方法

雄性成年 Wistar 大鼠分为四组。第一组作为对照，然而，第二和第三组接受菠萝蛋白酶 (250 mg/kg) 和 AlCl ₃ (34 mg/Kg, 1/25 LD ₅₀ )，第四组在 AlCl 前一小时补充菠萝蛋白酶₃、分别中毒。菠萝蛋白酶每天给药，AlCl ₃每隔一天通过口服管饲给药一个月。

结果

A1 中毒动物显示脂质过氧化（TBARS 和 H ₂ O ₂）水平和乳酸脱氢酶（LDH）活性升高。然而，降低的谷胱甘肽 (GSH) 和蛋白质含量、抗氧化酶 (SOD、CAT、GPx、GR、GST)、磷酸酶 (ALP、AcP) 和转氨酶 (AST、ALT) 活性显着降低。此外，还发现了激素水平（睾酮、促黄体生成素和促卵泡激素）和精子特征的显着变化。此外，检测到睾丸部分的组织学变异，这支持生化观察。否则，单独补充菠萝蛋白酶的大鼠会减少 TBARS 和 H ₂ O ₂并增加了大部分其他参数。此外，与铝处理组相比，在大鼠铝中毒之前补充菠萝蛋白酶在氧化应激标志物、激素和精子质量方面表现出值得改善的效果。

结论

总之，菠萝蛋白酶对铝诱导的睾丸功能障碍具有强大的保护作用，因此它代表了金属毒性处理的一种新方法。