The Spotted-wing Drosophila (Drosophila suzukii) is a devastating invasive pest of fruit crops. In D. melanogaster, the white (w) gene was associated with pigmentation and mating behavior, which are also important aspects to understand the invasion biology as well as to develop control strategies for D. suzukii. Here, we show that the generation of D. suzukii white-eyed mutants by CRISPR/Cas9 mutagenesis of the w gene resulted in the complete failure of copulation when w⁻ males were individually paired with w⁻ females in small circular arenas (diameter 0.7 cm) for 24 h. Further analysis showed that the mating defect was associated with w⁻ males and could not be rectified by two years of inbreeding by crossing sibling w⁻ females with w⁺ males, dim red illumination, male–female sexual training, changing to large arenas (diameter 3.5 cm), or different sex ratios. Profound pigmentation deficiency was detected in the compound eyes, ocelli, Malpighian tubules and testis sheaths in the w⁻ flies. Specifically, testis imaging showed that w⁻ males failed to deposit any pigments into pigment cells of the testis sheath, and produced smaller sperms and less seminal fluid compared to those from wildtype males. Together these observations suggest that the w gene plays an essential role in the regulation of sexual behavior and reproduction in D. suzukii. The similarities and differences in w gene function between D. suzukii and D. melanogaster in the context of pigmentation and mating behavior are discussed.

斑翅果蝇（Drosophila suzukii）是一种破坏性的水果作物入侵害虫。在D. melanogaster中，白色（w）基因与色素沉着和交配行为有关，这也是了解入侵生物学以及制定铃木D.控制策略的重要方面。在这里，我们表明，产生D. suzukii白眼突变体通过的CRISPR / Cas9突变W¯¯基因导致交配的彻底失败时，w ^ ^-男性分别用配对w ^ ^-女性在小圆形竞技场（直径0.7厘米）中放置24小时。进一步的分析表明，该配合缺陷与相关联的瓦特^-男性和不能两年近亲繁殖的通过杂交兄弟进行整流瓦特^-女瓦特⁺男性，暗淡红色照明，男女性训练，改变到大的舞台（直径3.5厘米）或其他性别比例。在复眼，单眼，马氏管检测深刻的色素沉着不足和睾丸鞘w ^ ^-苍蝇。具体而言，睾丸成像表明瓦特^-雄性未能将任何色素沉积到睾丸鞘的色素细胞中，与野生型雄性相比，产生的精子更小，精液更少。这些发现共同表明w基因在铃木D.的性行为和生殖的调节中起着至关重要的作用。在色素沉着和交配行为的背景下，讨论了铃木和黑腹果蝇w基因功能的异同。