In present study, we investigated the probiotic potential of three Pediococcus spp. isolated from Iranian traditional fermented cereal-dairy product, Tarkhineh. These 3 strains were identified as Pediococcus acidilactici VKU2, P. acidilactici IAH-5 and P. pentosaceus DHR005 by 16S rRNA gene sequencing. All the strain were found tolerate to pH 3 and 0.3% oxall for 3 h as well as simulated gastric and intestinal juice. P. acidilactici IAH-5 showed the highest cholesterol removal (67.52%), hydroxyl radical scavenging activity (58.32%), hydrophobicity (40.3%) and auto-aggregation (48%). Pediococcus spp. inhibited the growth of tested pathogens (Escherichia coli ATCC 25922, Pseudomonas aeruginosa PTCC 1707, Salmonella typhimurium PTCC 1609, and Staphylococcus aureus ATCC 25923) which the most susceptible strain was S. aureus. In competition assay, P. acidilactici IAH-5 was able to inhibited adhesion of 67.3% of S. typhimurium and in inhibition assay 45.8% of the pathogenic adhesion to Caco-2 cells were decreased. P. acidilactici VKU2 and P. acidilactici IAH-5 showed 16.32 and 12.25% adhesion to simulated epithelial cell line which were also confirmed by scanning electron microscopy. Pediococcus spp. did not showed DNase production or hemolytic activity which confirm its safety aspects. Our findings suggested that the P. acidilactici IAH-5 has the best properties with probiotic features and cholesterol assimilation for its application as novel bio-therapeutic and bio-preservation agents.

在本研究中，我们调查了三种Pediococcus spp的益生菌潜力。分离自伊朗传统的发酵谷物乳制品Tarkhineh。通过16S rRNA基因测序，将这3个菌株鉴定为乳酸双球菌VKU2，乳酸双球菌IAH-5和戊糖假单胞菌DHR005。发现所有菌株均耐受3小时的pH值和0.3％的奥沙尔以及模拟的胃液和肠液。乳酸假单胞菌IAH-5表现出最高的胆固醇去除率（67.52％），羟自由基清除活性（58.32％），疏水性（40.3％）和自聚集性（48％）。小球菌属。抑制测试病原体的生长（大肠杆菌ATCC 25922，铜绿假单胞菌PTCC 1707，鼠伤寒沙门氏菌PTCC 1609和金黄色葡萄球菌（ ATCC 25923），其中最易感的菌株是金黄色葡萄球菌。在竞争试验中，嗜酸乳酸杆菌IAH-5能够抑制鼠伤寒沙门氏菌67.3％的粘附，在抑制试验中，与Caco-2细胞的致病性粘附降低了45.8％。乳酸片球菌VKU2和乳酸片球菌IAH-5表现出16.32和12.25％的粘附性的模拟的上皮细胞系，其也通过扫描电子显微镜证实。小球菌spp。没有显示DNase产生或溶血活性，这证实了其安全性。我们的发现表明，乳酸乳酸杆菌IAH-5具有最佳的益生菌特性和胆固醇吸收特性，可作为新型生物治疗剂和生物防腐剂应用。