Despite the regenerative potential of the Peripheral Nervous System (PNS), injuries with loss of a nerve segment make the functional recovery a challenge. This work aimed to investigate the effects of the association of biodegradable conduits of poly (lactic acid) (PLA) with human adipose-derived stem cells (hADSCs) on the regeneration of the sciatic nerve. C57BL / 6 male mice were submitted to sciatic nerve transection followed by tubulization with PLA conduit. Animals were allocated in two groups: the first received an injection of DMEM inside the conduit (DMEM) and the second received hADSCs inside it (hADSC). Sensory and motor functions were assessed by the pinprick test and electroneuromiography, respectively. To assess neuronal survival the retrograde tracer fluorogold was injected into the sciatic nerve distally to the lesion site. One week after that, animals were sacrificed, tissues harvested and processed for morphological evaluation. After eight weeks, all animals showed sensory recovery in the pinprick test and there was no significant difference between the two groups. The amplitude of the compound muscle action potential was higher in the hADSCs group. The number of myelinated nerve fibers, muscle cells and motor plates was higher in the hADSC group. There was also greater survival of sensory and motor neurons in the hADSC animals. These results suggest that the association of PLA conduit and cell therapy with hADSCs leads to a better functional and morphological recovery after sciatic nerve transection.

尽管外周神经系统 (PNS) 具有再生潜力，但失去神经节段的损伤使功能恢复成为一项挑战。这项工作旨在研究可生物降解的聚乳酸 (PLA) 导管与人类脂肪干细胞 (hADSC) 的结合对坐骨神经再生的影响。C57BL / 6 雄性小鼠接受坐骨神经横断，然后用 PLA 导管进行小管化。动物被分为两组：第一组在导管内注射 DMEM (DMEM)，第二组在其中注射 hADSCs (hADSC)。分别通过针刺试验和神经电图评估感觉和运动功能。为了评估神经元存活率，将逆行示踪剂氟金注射到病变部位远端的坐骨神经中。一周后，处死动物，收获组织并进行形态学评估。八周后，所有动物在针刺试验中都表现出感觉恢复，两组之间没有显着差异。hADSCs 组复合肌肉动作电位的幅度较高。hADSC 组有髓神经纤维、肌肉细胞和运动板的数量较多。hADSC 动物的感觉和运动神经元的存活率也更高。这些结果表明 PLA 导管和细胞疗法与 hADSCs 的关联导致坐骨神经横断后更好的功能和形态恢复。所有动物在针刺试验中都表现出感觉恢复，两组之间没有显着差异。hADSCs 组复合肌肉动作电位的幅度较高。hADSC 组有髓神经纤维、肌肉细胞和运动板的数量较多。hADSC 动物的感觉和运动神经元的存活率也更高。这些结果表明 PLA 导管和细胞疗法与 hADSCs 的关联导致坐骨神经横断后更好的功能和形态恢复。所有动物在针刺试验中都表现出感觉恢复，两组之间没有显着差异。hADSCs 组复合肌肉动作电位的幅度较高。hADSC 组有髓神经纤维、肌肉细胞和运动板的数量较多。hADSC 动物的感觉和运动神经元的存活率也更高。这些结果表明 PLA 导管和细胞疗法与 hADSCs 的关联导致坐骨神经横断后更好的功能和形态恢复。hADSC 动物的感觉和运动神经元的存活率也更高。这些结果表明 PLA 导管和细胞疗法与 hADSCs 的关联导致坐骨神经横断后更好的功能和形态恢复。hADSC 动物的感觉和运动神经元的存活率也更高。这些结果表明 PLA 导管和细胞疗法与 hADSCs 的关联导致坐骨神经横断后更好的功能和形态恢复。