In this work, we designed at the level of microscale, subsurface flow wetlands planted with Typha latifolia, and used to remove diclofenac and naproxen from solutions that contained them. The wetlands were operated for 105 days with a hydraulic residence time (TRH) of 5 days. In these conditions, the removal efficiencies for diclofenac and naproxen were 98.4% and 97.7%, respectively. Moreover, we identified cultivable bacteria associated with the roots of plants exposed to either diclofenac or naproxen. We obtained 898 isolates that clustered in 9 morphotypes from the roots of Typha latifolia exposed to diclofenac, and 563 isolates grouped in 7 morphotypes, in the case of naproxen. All isolates were identified by 16S ribosomal sequencing. The BLASTn analysis indicated that 16 morphotypes showed an identity higher than 95% with the 16S rDNA gene of bacteria belonging to the genus Pseudomonas. Biochemical characterization based on plant growth-promoting activities suggests that bacteria contribute to plant growth in the wetland conditions. The results indicate that Typha latifolia and bacteria associated with their roots removed diclofenac and naproxen in subsurface flow wetlands.

中文翻译：

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人工湿地中与香蒲根相关的可培养细菌的分离，用于去除双氯芬酸或萘普生

在这项工作中，我们在种植有香蒲的微型地下渗流湿地上进行了设计，并用于从含有它们的溶液中去除双氯芬酸和萘普生。湿地运行105天，水力停留时间（TRH）为5天。在这些条件下，双氯芬酸和萘普生的去除效率分别为98.4％和97.7％。此外，我们确定了与暴露于双氯芬酸或萘普生的植物根部相关的可培养细菌。我们从香蒲的根部获得了以9种形态型聚类的898株如果是萘普生，则将其暴露于双氯芬酸，并将563种分离物分为7种形态型。通过16S核糖体测序鉴定所有分离物。BLASTn分析表明，与假单胞菌属细菌的16S rDNA基因相比，有16个形态型显示出高于95％的同一性。基于促进植物生长活性的生化特征表明，细菌有助于湿地条件下的植物生长。结果表明香蒲和与其根相关的细菌去除了地下流湿地中的双氯芬酸和萘普生。