The underlying principles influencing bacteria community assembly have long been of interest in the field of microbial ecology. Environmental heterogeneity is believed to be important in controlling the uniqueness and variability of communities. However, little is known about the influence of the host macrophytes on epiphytic bacteria assembly. In this study, we used two contrasting artificial water environments (eutrophic and oligotrophic) for reciprocal transplant experiment of Myriophyllum spicatum to examine the colonization of epiphytic bacteria accompanied with plants growth. Comparative analysis addressed a higher species diversity in epiphytic bacteria than in bacterioplankton, and the highest microbiome richness in sediment. Our data revealed that the organization of epiphytic bacterial community was affected by both plant status (i.e. branch number, net photosynthesis rate etc.) and water bodies (i.e. total phosphate, total nitrogen, pH etc.). Moreover, plant status effected the assembly in priority to water. 16S rRNA gene sequencing further indicated that the epiphytic assemblies were motivated by functionalization and interplay with hosts as a whole. The results complemented new evidences for the ‘lottery process’ in the epiphytic bacteria assembly traits and shed insights into the assembly patterns referring to functional adaptation across epiphytic bacteria and macrophytes.

中文翻译：

---

狐尾藻附生细菌集合的结构变异和功能预测

影响细菌群落组装的基本原理长期以来一直是微生物生态学领域的研究热点。环境异质性被认为在控制社区的独特性和可变性方面很重要。然而，关于宿主大型植物对附生细菌组装的影响知之甚少。在这项研究中，我们使用两种对比鲜明的人工水环境（富营养化和贫营养化）进行了狐尾藻的相互移植实验，以检查伴随植物生长的附生细菌的定植。比较分析表明，附生细菌的物种多样性高于浮游细菌，沉积物中微生物组的丰富度最高。我们的数据显示，附生细菌群落的组织受两种植物状态（即 支数、净光合速率等）和水体（即总磷酸盐、总氮、pH 值等）。此外，植物状态影响装配优先于水。16S rRNA 基因测序进一步表明附生组件是由功能化和与宿主相互作用的整体驱动的。结果补充了附生细菌组装性状中“抽签过程”的新证据，并深入了解了涉及附生细菌和大型植物的功能适应的组装模式。16S rRNA 基因测序进一步表明附生组件是由功能化和与宿主相互作用的整体驱动的。结果补充了附生细菌组装性状中“抽签过程”的新证据，并深入了解了涉及附生细菌和大型植物的功能适应的组装模式。16S rRNA 基因测序进一步表明附生组件是由功能化和与宿主相互作用的整体驱动的。结果补充了附生细菌组装性状中“抽签过程”的新证据，并深入了解了涉及附生细菌和大型植物的功能适应的组装模式。