We studied the effect of turmeric extract in the composition of rectal suppositories on the level of LPO products and oxidative modification of proteins in the colon mucosa of Wistar rats with experimental Crohn’s disease modeled by rectal administration of trinitrobenzenesulfonic acid. The suppositories containing turmeric extract were administered 12 h after disease induction. On days 3, 5, and 7 of the experiment, clinical parameters of the disease were scored using disease activity scale (DAI) and the concentration of LPO products and intensity of oxidative modification of proteins were measured by the extraction-spectrofluorimetric method. Administration turmeric extract in rectal suppositories reduced the severity of clinical symptoms, the level of LPO products (mostly in the isopropanol phase of the lipid extract), and the total content of products of oxidative modification of proteins. Moreover, correlations between DAI and concentration of LPO products in the colon were found.

中文翻译：

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姜黄提取物在实验性克罗恩病原始成分直肠栓中的抗氧化作用

我们研究了姜黄提取物在直肠栓剂组合物中对通过直肠给药三硝基苯磺酸模拟的患有实验性克罗恩病的 Wistar 大鼠结肠黏膜中 LPO 产物水平和蛋白质氧化修饰的影响。在疾病诱导后 12 小时施用含有姜黄提取物的栓剂。在实验的第 3、5 和 7 天，使用疾病活动量表 (DAI) 对疾病的临床参数进行评分，并通过提取-荧光光谱法测量 LPO 产物的浓度和蛋白质的氧化修饰强度。在直肠栓剂中使用姜黄提取物可降低临床症状的严重程度、LPO 产物的水平（主要在脂质提取物的异丙醇相中），以及蛋白质氧化修饰产物的总含量。此外，还发现了 DAI 与结肠中 LPO 产物浓度之间的相关性。