Genus Vicia is a member of family Fabaceae and comprises 180 to 210 species. The most important species is faba bean (Vicia faba) which is still one of the most favourable grain legumes over all the world. The genus contains some additional food crops and a number of forage plants and some other weedy strains such as Vicia angustifolia and Vicia cordata. The aim of the present investigation is to elucidate the phylogenetic relationships among four Vicia species, two species (Vicia angustifolia L. ssp. Angustifolia (2n = 12) and Vicia cordata wulfen ex Hoppe (2n = 10)) belong to section Vicia, Vicia dalmatica A. Kern (2n = 12, section Cracca), and Vicia johannis tamamsch (2n = 14, section Faba). Two tools have been applied to identify the genetic relationships among the examined species, double fluorescence in situ hybridization (FISH) has been used to localize the sites of 5S and 45S rDNA, and sodium dodecyl sulfate-poly acrylamide gel electrophoretic (SDS-PAGE) patterns of total seed storage protein fractions. Double FISH experiment has not shown any variation in the loci number, but the positions along the chromosomes were different; both Vicia johannis and Vicia dalmatica exhibited the same interstitial 45S rRNA gene loci, while Vicia angustifolia and Vicia cordata have shown single large stretched 45S rRNA loci almost at the terminal region of the shortest chromosome. It could be concluded from the similarity matrix among the Vicia species as computed according to Jaccard coefficient from the SDS-PAGE, that V. cordata is similar to V. angustifolia and V. dalmatica by a percentage of 73 and 69%, respectively, and the most related species to V. johannis is V. dalmatica (~ 64%). FISH and SDS-PAGE of the total seed storage proteins together reflected the similar genetic relationship among the studied species as fellows, V. angustifolia is more related to V. cordata then comes V. dalmatica and then V. johannis which is at a distal position from the other species.

中文翻译：

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利用FISH和总种子贮藏蛋白模式研究某些蚕豆的遗传关系。

蚕豆属是豆科的成员，包括180至210种。最重要的物种是蚕豆（蚕豆），它仍然是全世界最有利的谷类豆类之一。该属还包含一些其他的粮食作物和一些饲用植物以及一些其他杂草菌株，例如野豌豆和野豌豆。本研究的目的是阐明属于野豌豆属野豌豆科的四种野豌豆属植物的亲缘关系，其中两种是野豌豆（野豌豆（Anciaifolia L. ssp。Angustifolia，2n = 12）和野豌豆（Hoppe）（2n = 10））。 dalmatica A. Kern（2n = 12，克拉卡地区）和Vicia johannis tamamsch（2n = 14，法巴地区）。已经使用了两种工具来确定所检查物种之间的遗传关系，双荧光原位杂交（FISH）已用于定位5S和45S rDNA的位点，以及总种子存储蛋白级分的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）模式。双重FISH实验未显示出基因座数目的任何变化，但是沿染色体的位置是不同的。野豌豆和野豌豆均表现出相同的间质45S rRNA基因位点，而野豌豆和野豌豆则在几乎最短染色体的末端区域都显示出一个大的拉伸45S rRNA基因座。根据根据SDS-PAGE的Jaccard系数计算得出的野豌豆物种之间的相似性矩阵可以得出结论，堇青菜与蛤an和达尔马蒂草的相似度分别为73％和69％，与V. johannis最相关的物种是V. dalmatica（〜64％）。全部种子贮藏蛋白的FISH和SDS-PAGE共同反映了所研究物种之间的相似遗传关系，即同种植物（V. angustifolia）与V. cordata的相关性更高，然后是V. dalmatica，然后是V. johannis，其位于远端位置来自其他物种。