Plant growth promoting bacteria (PGPB) are a growing subset of agricultural adjuncts which can be used to increase crop yield and plant productivity. Although substantial research has been conducted on the metabolites and active molecules secreted by PGPBs, relatively little is known about their effects on the global transcriptome of the host plant. The present study was carried out to investigate changes in the gene expression landscape of early vegetative Brassica napus following treatment with Pseudomonas chlororaphis PA23. This PGPB was isolated from the soybean rhizosphere and has been extensively studied as a biocontrol agent. However, little is known about its effects on plant growth and development. Using a combination of RNA-sequencing and physiological analyses, we identified increased abundance of mRNA transcripts associated with photosynthesis and phytohormone response. Phenotypically we observed increased photosynthetic rates and larger root and shoot systems in B. napus following P. chlororaphis PA23 treatment. Lastly, we identified auxin production by P. chlororaphis PA23 which likely contributes to changes in gene expression and the observed phenotypic differences in root and shoot structures. Together, the results of our study suggest that PA23 is a potent plant growth promoting agent with the potential for field applications as an agricultural adjunct.

中文翻译：

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全局RNA测序揭示了用假单胞菌PA23处理的甘蓝型油菜光合作用活性和生长素应答增强

促进植物生长的细菌（PGPB）是农业助剂的一个增长子集，可用于提高作物产量和植物生产力。尽管已对PGPB分泌的代谢物和活性分子进行了大量研究，但对它们对宿主植物整体转录组的影响了解甚少。本研究旨在调查用绿假单胞菌PA23处理的早期营养甘蓝型油菜的基因表达格局的变化。该PGPB是从大豆根际中分离出来的，已作为生物防治剂进行了广泛的研究。然而，对其对植物生长和发育的影响知之甚少。结合RNA测序和生理分析，我们发现与光合作用和植物激素反应相关的mRNA转录物的丰度增加。从表型上看，我们观察到，在甘蓝假单胞菌PA23处理后，甘蓝型油菜的光合作用速率增加，根系和芽系统增大。最后，我们确定了P. chlororaphis PA23产生的植物生长素，这可能有助于基因表达的变化以及观察到的根和茎结构的表型差异。总之，我们的研究结果表明，PA23是一种有效的植物生长促进剂，具有作为农业辅助剂在田间应用的潜力。叶绿素PA23可能有助于基因表达的变化以及在根和茎结构中观察到的表型差异。总之，我们的研究结果表明PA23是一种有效的植物生长促进剂，具有作为农业辅助剂在田间应用的潜力。叶绿素PA23可能有助于基因表达的变化以及在根和茎结构中观察到的表型差异。总之，我们的研究结果表明PA23是一种有效的植物生长促进剂，具有作为农业辅助剂在田间应用的潜力。