ABSTRACT

We researched clinically relevant antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in environmental samples from a recreation club in Brazil. A total of 172 amplicons (105 from soil and 67 from water) of 26 ARGs (20 among the soil and water samples; four only in soil samples; two only in water samples) were detected. Nine MGEs were detected, including plasmids and class 1 integron. The absolute abundance of the mcr-3 gene ranged from 1.12 × 10² to 1.81 × 10³ copies/mL⁻¹ in water samples. The rapid spread of mcr-like genes in several sources has generated a huge concern to public health. Accordingly, understanding of antimicrobial resistance, carry out surveillance studies may contribute to tackle antimicrobial resistance. As the environmental samples were collected from a popular recreation club in Brazil, this study points out to the risk and exposure to clinically relevant ARGs, especially to mcr-3 and mcr-7.1 genes.

摘要

我们研究了巴西一家娱乐俱乐部的环境样本中临床相关的抗菌素耐药性基因 (ARGs) 和移动遗传元件 (MGEs)。共检测到 26 个 ARGs 的 172 个扩增子（105 个来自土壤，67 个来自水）（土壤和水样中的 20 个；仅土壤样品中的 4 个；仅水样中的两个）。检测到九个 MGE，包括质粒和 1 类整合子。水样中mcr-3基因的绝对丰度范围为 1.12 × 10 ²到 1.81 × 10 ³拷贝/mL ^-1。mcr的迅速普及几个来源的类基因已经引起了公众健康的巨大关注。因此，了解抗菌素耐药性、开展监测研究可能有助于解决抗菌素耐药性问题。由于环境样本是从巴西一家受欢迎的娱乐俱乐部收集的，这项研究指出了与临床相关的 ARGs 的风险和暴露，尤其是mcr-3和mcr-7.1基因。