In the present research, the eggs from four rainbow trout females were used to provide four groups of gynogenetic doubled haploids (DHs). The quality of the eggs from different clutches was comparable, however, interclutch differences were observed in the gynogenetic variants of the experiment and the survival of DH specimens from different groups varied from 3% to 57% during embryogenesis. Transcriptome analysis of the eggs from different females exhibited inter-individual differences in the maternal genes’ expression. Eggs originating from females whose gynogenetic offspring had the highest survival showed an increased expression of 46 genes when compared to the eggs from three other females. Eggs with the highest survival of gynogenetic embryos showed an up-regulation of genes that are associated with cell survival, migration and differentiation (tyrosine-protein kinase receptor TYRO3-like gene), triglyceride metabolism (carnitine O-palmitoyltransferase 1 gene), biosynthesis of polyunsaturated fat (3-oxoacyl-acyl-carrier-protein reductase gene), early embryogenic development (protein argonaute-3 gene, leucine-rich repeat-containing protein 3-like gene), 5S RNA binding (ribosome biogenesis regulatory protein homolog) as well as senescence and aging (telomerase reverse transcriptase, TERT gene), among others. Positive correlation between the genotypic efficiency and egg transcriptome profiles indicated that at least some of the differentially expressed genes should be considered as potential candidate genes for the efficiency of gynogenesis in rainbow trout.

中文翻译：

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基于比较转录组分析，与雌雄同体虹鳟（Oncorhynchus mykiss）的存活相关的候选基因的初步鉴定。

在本研究中，来自四只虹鳟雌性的卵被用来提供四组雌激素双倍单倍体（DHs）。来自不同离合器的卵的质量是可比较的，但是，在实验的雌激素发育变体中观察到了离合器之间的差异，并且在胚胎发生期间，来自不同组的DH标本的存活率从3％到57％不等。来自不同雌性卵的转录组分析显示出母本基因表达的个体差异。与其他三只雌性的卵相比，雌性生殖后代存活率最高的雌性卵显示增加了46个基因的表达。雌成虫胚胎存活率最高的卵显示与细胞存活率相关的基因上调，迁移和分化（酪氨酸蛋白激酶受体TYRO3样基因），甘油三酸酯代谢（肉碱O-棕榈酰转移酶1基因），多不饱和脂肪的生物合成（3-氧代酰基-酰基-载体蛋白还原酶基因），早期胚胎发育（蛋白argonaute） -3基因，含亮氨酸的重复序列丰富的蛋白3-like基因），5S RNA结合（核糖体生物发生调节蛋白同源物）以及衰老和衰老（端粒酶逆转录酶，TERT基因）等。基因型效率和卵转录组谱之间的正相关表明，至少一些差异表达的基因应被视为虹鳟鱼雌核发育效率的潜在候选基因。甘油三酸酯代谢（肉碱O-棕榈酰转移酶1基因），多不饱和脂肪的生物合成（3-氧代酰基酰基载体蛋白还原酶基因），早期胚胎发育（蛋白argonaute-3基因，富含亮氨酸的重复序列蛋白3-like基因） ），5S RNA结合（核糖体生物发生调节蛋白同源物）以及衰老和衰老（端粒酶逆转录酶，TERT基因）等。基因型效率和卵转录组谱之间的正相关表明，至少一些差异表达的基因应被视为虹鳟鱼雌核发育效率的潜在候选基因。甘油三酸酯代谢（肉碱O-棕榈酰转移酶1基因），多不饱和脂肪的生物合成（3-氧代酰基酰基载体蛋白还原酶基因），早期胚胎发育（蛋白argonaute-3基因，富含亮氨酸的重复序列蛋白3-like基因） ），5S RNA结合（核糖体生物发生调节蛋白同源物）以及衰老和衰老（端粒酶逆转录酶，TERT基因）等。基因型效率和卵转录组谱之间的正相关表明，至少一些差异表达的基因应被视为虹鳟鱼雌核发育效率的潜在候选基因。富含亮氨酸的重复序列含蛋白3样基因），5S RNA结合（核糖体生物发生调节蛋白同源物）以及衰老和衰老（端粒酶逆转录酶，TERT基因）等。基因型效率和卵转录组谱之间的正相关表明，至少一些差异表达的基因应被认为是虹鳟鱼雌核发育效率的潜在候选基因。富含亮氨酸的重复序列含蛋白3样基因），5S RNA结合（核糖体生物发生调节蛋白同源物）以及衰老和衰老（端粒酶逆转录酶，TERT基因）等。基因型效率和卵转录组谱之间的正相关表明，至少一些差异表达的基因应被视为虹鳟鱼雌核发育效率的潜在候选基因。