In the present study, the concentration of decorin in canine normal and neoplastic mammary gland tissues was examined to understand the potential role of decorin in development and progression of canine mammary tumours. The homogenates of 48 mammary gland tumours (10 benign and 38 malignant) and 10 samples of normal canine mammary gland tissue were used in the study. The presence and quantification of decorin was examined in the homogenates using Western blot and specific canine ELISA. Western blotting confirmed the presence of decorin both in the normal mammary gland tissues and in the mammary gland tumours. The concentration of decorin was significantly higher (p < .05) in the benign tumours and non‐metastatic malignant tumours than in the normal mammary gland. The concentration of decorin was significantly lower (p < .05) in the malignant tumours with metastasis to regional lymph nodes compared with benign tumours and non‐metastatic malignant tumours. No significant differences were found in the level of decorin between the benign and the non‐metastatic malignant tumours. Both the histological type of malignant tumours and the histological grade did not significantly affect the concentration of decorin. These findings suggest that neoplastic transformation in the canine mammary gland leads to increase in the decorin protein synthesis. The reducing decorin concentration in canine malignant mammary tumours appears to facilitate the metastatic spread of these tumours.

中文翻译：

---

犬正常和赘生性乳腺组织中的Decorin浓度

在本研究中，检查了犬正常和肿瘤性乳腺组织中香精蛋白的浓度，以了解香精蛋白在犬乳腺肿瘤发生发展中的潜在作用。本研究使用了48例乳腺肿瘤（10例良性和38例恶性）的匀浆和10例正常犬乳腺组织样本。使用蛋白质印迹法和特异性犬ELISA法检查匀浆中香精蛋白的存在和定量。蛋白质印迹证实了正常乳腺组织和乳腺肿瘤中都存在decorin。核心蛋白聚糖的浓度显着较高（p <.05），良性肿瘤和非转移性恶性肿瘤的发生率要比正常乳腺高。 与良性肿瘤和非转移性恶性肿瘤相比，在转移至区域淋巴结的恶性肿瘤中，decorin的浓度显着降低（p <.05）。良性和非转移性恶性肿瘤之间的除蛋白水平没有显着差异。恶性肿瘤的组织学类型和组织学等级均未显着影响除蛋白的浓度。这些发现表明，犬乳腺中的肿瘤转化导致了核心蛋白聚糖蛋白合成的增加。犬恶性乳腺肿瘤中decorin浓度的降低似乎促进了这些肿瘤的转移扩散。