World climate change has triggered soil water stress and imposed limitations on agricultural production. Plant growth-promoting bacteria (PGPBs) have been an efficient strategy to improve the biological supply and growth of plants under distinct abiotic stress conditions. We hypothesized that the soils from a temporary pond may harbor PGPBs with potential strains which increase maize tolerance to water deficit. We studied rhizosphere and bulk soil of Mimosa bimucronata in a temporary pond from semiarid Northeast Brazil to access strains with characteristics to promote plant growth and mitigate abiotic stress for maize crop. We isolated 355 bacterial isolates, from which 96 were selected based on the morphophysiological characterization to assess IAA production (42 % produced over 50 μg mL^-1 of IAA), calcium phosphate solubilization (with one isolate achieving medium IS), biofilm and exopolysaccharides production (66 % and 98 % of isolates, respectively). Based on these mechanisms, the 30 most promising bacterial isolates were selected to assess biological nitrogen fixation (74 % of the isolates showed nitrogenase activity greater than 20 C₂H₄.h^-1.mg^-1), ACC deaminase activity (80 % of isolates) and growth in medium with reduced water activity (8 % of isolates grew in medium with water activity (A_w) of 0.844). We sequenced the 16S rRNA gene from the seven most promising isolates in in vitro and in vivo assays, which were identified as Staphylococcus edaphicus, Bacillus wiedmannii, Micrococcus yunnanensis, Streptomyces alboflavus, Streptomyces alboflavus, Bacillus wiedmanni and Bacillus cereus. In vivo, eleven isolates and three bacterial consortia did not differ from the control with nutrient solution, for total leaf area and root dry mass of maize. S. alboflavus (BS43) had the best in vivo results, not differing from the control with nutrient solution. We highlight the unpublished potential of Staphylococcus edaphicus and Streptomyces alboflavus in promoting the growth of plants under water stress. In addition, it is the first report of bacteria isolated from a temporary pond in the Brazilian semiarid which promoting plant growth attributes and development.

世界气候变化引发了土壤水分压力，并限制了农业生产。促进植物生长的细菌（PGPBs）是一种有效的策略，可以在不同的非生物胁迫条件下改善植物的生物供应和生长。我们假设临时池塘的土壤可能含有PGPB，潜在的菌株可能增加玉米对水分亏缺的耐受性。我们在巴西东北部半干旱的一个临时池塘中研究了含羞草的根际和散装土壤，以获取具有促进植物生长和减轻玉米作物非生物胁迫特性的菌株。我们分离了355个细菌分离株，根据形态生理特征从中选择了96个，以评估IAA的产生（超过50μgmL ^-1产生42％IAA），磷酸钙增溶（一种分离物达到中等IS），生物膜和胞外多糖的产生（分别占分离物的66％和98％）。基于这些机制，选择了30种最有前途的细菌分离株以评估生物固氮（74％的分离株显示的固氮酶活性大于20 C ₂ H ₄ .h ^-1 .mg ^-1），ACC脱氨酶活性（80％分离株）和在水分活度降低的培养基中生长（8％分离株在水分活度（A _w）为0.844的培养基中生长）。我们在体外和体内试验中从七个最有希望的分离株中测序了16S rRNA基因。金edaphicus，wiedmannii芽孢杆菌，微云南松，链霉菌alboflavus，链霉菌alboflavus，芽孢杆菌wiedmanni和蜡样芽孢杆菌。在体内，对于玉米的总叶面积和根干质量，11个分离株和3个细菌菌群与使用营养液的对照没有差异。S. alboflavus（BS43）在体内的效果最好，与营养液的对照无差异。我们强调了葡萄球菌和链霉菌的未发表潜力促进水分胁迫下植物的生长。此外，这是从巴西半干旱地区的一个临时池塘中分离出的细菌的首次报道，该细菌促进植物的生长特性和发育。