Penaeid prawns are considered as most demanding fishery resources. The current study aims to purify and characterize lectin from the haemolymph of Penaeus semisulcatus. The semisulcatus-lectin was purified by affinity chromatography using mannose coupled Sepharose CL-4B column and purified lectin exhibited a single band of 66 kDa in SDS-PAGE. The purity and crystalline structure of purified lectin was confirmed by HPLC and X-ray diffraction analysis. Semisulcatus-lectin exhibited yeast agglutination activity against Saccharomyces cerevisiae and agglutinated human erythrocytes. Semisulcatus-lectin was evaluated for phenol oxidase activation and phagocytic activities. It was observed that semisulcatus-lectin had antibacterial activity against Gram-negative Vibrio parahaemolyticus and Aeromonas hydrophila, suggesting a potential therapeutic strategy in aquaculture industry for disease management.

对虾被认为是最苛刻的渔业资源。目前的研究目的是从半边对虾的血淋巴中纯化和鉴定凝集素。的短沟-凝集素通过使用甘露糖亲和层析纯化偶联的Sepharose CL-4B柱纯化凝集素在SDS-PAGE显示出66 kDa的单一带。通过HPLC和X射线衍射分析确认纯化的凝集素的纯度和晶体结构。半硫化物-凝集素对酵母菌和凝集的人红细胞表现出酵母凝集活性。评估半硫化物-凝集素的酚氧化酶活化和吞噬活性。据观察，semisulcatus-lectin对革兰氏阴性副溶血性弧菌和嗜水气单胞菌具有抗菌活性，表明在水产养殖业用于疾病管理的潜在治疗策略。