Abstract

We developed an all-solid-state sensor for monoascorbate ion based on a composite containing copper(II) ions (5 wt %), graphite and epoxy resin. The sensor presented super-Nernstian response at room temperature under continuous bubbling of oxygen gas in sample. The detection limit of the sensor was 0.02 mM and its linear range was between 0.05 and 1.5 mM with upper limit of quantification equal to 3 mM. The leaching out effect of the active component from composite was estimated less than 11 µg of copper(II) sulfate per day under continuous diffusion process in deionized water. Photomicrographs of the electrode surface did not show the presence of salt particles on the surface indicating that copper(II) ions could be intercalated in graphene layers. Attenuated total reflectance infrared spectrum demonstrated this possibility. The sensor was successfully applied to the in vitro determination of L-ascorbic acid in urine.

中文翻译：

---

基于铜离子复合物的电位传感器测定尿液中的维生素C

摘要

我们开发了一种基于包含铜离子（5％），石墨和环氧树脂的复合材料的单抗坏血酸离子全固态传感器。传感器在样品中不断冒泡的氧气下，在室温下表现出超能斯特响应。传感器的检出限为0.02 mM，线性范围为0.05至1.5 mM，定量上限为3 mM。据估计，在去离子水中连续扩散过程中，活性成分从复合物中的浸出效果每天少于11 µg硫酸铜（II）。电极表面的显微照片未显示盐颗粒在表面上的存在，表明铜（II）离子可插入石墨烯层中。衰减的全反射红外光谱证明了这种可能性。