Determination of Quisqualic Acid in quisqualis fructus by Pre-column Derivatization and High Performance Liquid Chromatography,Journal of Analytical Chemistry

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Determination of Quisqualic Acid in quisqualis fructus by Pre-column Derivatization and High Performance Liquid Chromatography
Journal of Analytical Chemistry ( IF 1.1 ) Pub Date : 2020-07-31 , DOI: 10.1134/s1061934820080055
Siyuan Chang , Bingfeng Li , Tianyi Chen , Xuejun He , Bin Wu

Abstract

A convenient, cheap and accurate method for the determination of quisqualic acid in traditional Chinese medicine Quisqualis fructus by pre-column derivatization with 2-nitrobenzenesulfonyl chloride and HPLC was established. Quisqualis fructus samples were homogenised in water after being crushed and degreased with petroleum ether. Target compounds were separated and detected on a C18 column using gradient elution. The recovery rates were in the range of 90–103%. Good linearity within the range of 0.2–20 mg/mL for quisqualic acid with correlation coefficients of ≥0.98 was observed. The relative standard deviations (RSDs) of the within-day and between-day retention time precisions were 0.15–0.17% and 0.8–0.9%, respectively. The RSDs of the within-day and between-day integrated area precisions were 2.3–6.4% and 4.8–9.8%, respectively. The concentrations of quisqualic acid (1.5 mg/g) from Quisqualis fructus were conveniently measured without any expensive photodiode array detector but with a common UV–visible detector.

更新日期：2020-07-31

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