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Gut bacteria of Varanus salvator possess potential antitumour molecules.
International Microbiology ( IF 3.1 ) Pub Date : 2020-07-31 , DOI: 10.1007/s10123-020-00139-9
Morhanavallee Soopramanien 1 , Naveed Ahmed Khan 2 , Kuppusamy Sagathevan 1 , Ruqaiyyah Siddiqui 2
Affiliation  

Pollution, unhygienic conditions and organic waste are detrimental to human health. On the contrary, animals living in polluted environments, feeding on organic waste and exposed to noxious agents such as heavy metals must possess remarkable properties against contracting diseases. Species such as cockroaches and water monitor lizards thrive in unhygienic conditions and feed on decaying matter. Here, we investigated the antitumour properties of metabolites produced by gut bacteria isolated from Varanus salvator (Asian water monitor lizard). An adult water monitor lizard and a juvenile water monitor lizard were acquired, and dissected. Their aerobic gut bacteria were isolated and identificated through 16S rDNA sequencing. Next, bacterial conditioned media (CM) were prepared and utilised for subsequent assays. Growth inhibition, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell viability assay, cytotoxicity and cell survival assays were accomplished against a panel of cancer cells as well as a normal cell line. Furthermore, liquid chromatography-mass spectrometry (LC-MS) was employed to identify potential antitumour molecules. A plethora of bacteria were isolated from the gut of juvenile and adult V. salvator respectively. Moreover, CM prepared from selected bacteria exhibited antitumour activity. Of note, LC-MS results indicated the presence of several molecules with reported antitumour activity, namely, 3-butylidene-7-hydroxyphthalide, C75, enigmol, estrone 16-oxime, proglumide and S-allyl-l-cysteine. Furthermore, 356 potentially novel molecules from juvenile V. salvator and 184 from adult V. salvator were depicted. Thus, the gut microbiota of V. salvator might be considered as a great niche of antitumour molecules; however, further in vitro and in vivo studies are needed to assess the antitumour properties of these molecules.



中文翻译:

巨蜥的肠道细菌具有潜在的抗肿瘤分子。

污染、不卫生的条件和有机废物对人类健康有害。相反,生活在污染环境中、以有机废物为食并接触重金属等有毒物质的动物必须具有显着的抗感染能力。蟑螂和水蜥蜴等物种在不卫生的条件下茁壮成长,并以腐烂的物质为食。在这里,我们研究了从Varanus salvator 中分离的肠道细菌产生的代谢物的抗肿瘤特性(亚洲水巨蜥)。获得一只成年水巨蜥和一只幼年水巨蜥,并对其进行解剖。他们的需氧肠道细菌通过 16S rDNA 测序被分离和鉴定。接下来,制备细菌条件培养基 (CM) 并用于后续检测。针对一组癌细胞以及正常细胞系完成了生长抑制、MTT 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物细胞活力测定、细胞毒性和细胞存活测定。此外,液相色谱-质谱(LC-MS)被用来鉴定潜在的抗肿瘤分子。从幼年和成年V. salvator的肠道中分离出大量细菌分别。此外,从选定的细菌制备的 CM 表现出抗肿瘤活性。值得注意的是,LC-MS 结果表明存在几种具有抗肿瘤活性的分子,即 3-丁叉基-7-羟基苯酞、C75、enigmol、雌酮 16-肟、丙谷胺和 S-烯丙基-1-半胱氨酸。此外,还描绘了来自幼年V. salvator 的356 个潜在新分子和来自成年V. salvator 的184 个。因此,V. salvator的肠道微生物群可能被认为是抗肿瘤分子的一个重要生态位。然而,需要进一步的体外和体内研究来评估这些分子的抗肿瘤特性。

更新日期:2020-07-31
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