Abstract

Serratia marcescens, rod-shaped Gram-negative bacteria is classified as an opportunistic pathogen in the family Enterobacteriaceae. It causes a wide variety of infections in humans, including urinary, respiratory, ocular lens and ear infections, osteomyelitis, endocarditis, meningitis and septicemia. Unfortunately, over the past decade, antibiotic resistance has become a serious health care issue; the effective means to control and dissemination of S. marcescens resistance is the need of hour. The whole genome sequencing of S. marcescens FGI94 strain contains 4434 functional proteins, among which 690 (15.56%) proteins were classified under hypothetical. In the present study, we applied the power of various bioinformatics tools on the basis of protein family comparison, motifs, functional properties of amino acids and genome context to assign the possible functions for the HPs. The pseudo sequences (protein sequence that contain ≤100 amino acid residues) are eliminated from the study. Although we have successfully predicted the function for 483 proteins, we were able to infer the high level of confidence only for 108 proteins. The predicted HPs were classified into various classes such as enzymes, transporters, binding proteins, cell division, cell regulatory and other proteins. The outcome of the study could be helpful to understand the molecular mechanism in bacterial pathogenesis and also provide an insight into the identification of potential targets for drug and vaccine development.

中文翻译：

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粘质沙雷氏菌FGI94的假设蛋白的计算机功能注释和表征。

摘要

粘质沙雷氏菌（Serratia marcescens），杆状革兰氏阴性细菌在肠杆菌科中被分类为机会病原体。它引起人类多种感染，包括尿，呼吸道，眼晶状体和耳朵感染，骨髓炎，心内膜炎，脑膜炎和败血病。不幸的是，在过去的十年中，抗生素耐药性已成为一个严重的医疗保健问题。控制和传播马氏链球菌抗性的有效手段是需要小时。马氏链球菌的全基因组测序FGI94菌株包含4434个功能蛋白，其中690（15.56％）个蛋白被假设为分类。在本研究中，我们基于蛋白质家族比较，基序，氨基酸的功能特性和基因组背景应用了各种生物信息学工具的功能，以为HP分配可能的功能。该研究消除了伪序列（包含≤100个氨基酸残基的蛋白质序列）。尽管我们已经成功地预测了483种蛋白质的功能，但是我们只能推断出108种蛋白质的高置信度。预测的HP分为各种类别，例如酶，转运蛋白，结合蛋白，细胞分裂，细胞调节蛋白和其他蛋白。