Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Graphene-Based Hall Effect Biosensor for Improved Specificity and Sensitivity of Label-Free DNA Detection
Nano ( IF 1.2 ) Pub Date : 2020-05-15 , DOI: 10.1142/s1793292020500885 Naiyuan Cui 1 , Fei Wang 1 , Hanyuan Ding 1
Nano ( IF 1.2 ) Pub Date : 2020-05-15 , DOI: 10.1142/s1793292020500885 Naiyuan Cui 1 , Fei Wang 1 , Hanyuan Ding 1
Affiliation
There is a broad interest in using graphene or graphene oxide (GO) sheets as a transducer for selective and label-free detection of biomolecules such as DNA, tumor marker, biological ions, etc. Here, a chemical vapor deposition (CVD) graphene-based Hall effect biosensor used for ultrasensitive label-free detection of DNA via DNA hybridization is reported. Hall effect measurements based on the Van der Pauw method are used to perform single-base sequence selective detection of DNA on graphene sheets, which are prepared by CVD. The mobility decreases and the sheet resistance increases with the adding of either complementary or one-base mismatched DNA to the graphene device. The hole carrier concentration of the graphene devices increases apparently with the addition of complementary DNA while it is hardly affected by the one-base mismatched DNA. The detection limit as low as 1[Formula: see text]pM was realized with a linear range from 1[Formula: see text]pM to 100[Formula: see text]nM. Moreover, the Hall effect biosensor was able to distinguish the complementary DNA from one-base mismatched DNA with a high specificity of [Formula: see text]6.2 which is almost two orders of magnitude higher than that of the previously reported graphene biosensors based on DNA–DNA hybridization.
中文翻译:
用于提高无标记 DNA 检测的特异性和灵敏度的基于石墨烯的霍尔效应生物传感器
人们广泛关注使用石墨烯或氧化石墨烯 (GO) 片材作为传感器,用于选择性和无标记检测生物分子,例如 DNA、肿瘤标志物、生物离子等。这里,化学气相沉积 (CVD) 石墨烯-报道了一种基于霍尔效应的生物传感器,用于通过 DNA 杂交对 DNA 进行超灵敏的无标记检测。基于 Van der Pauw 方法的霍尔效应测量用于对通过 CVD 制备的石墨烯片上的 DNA 进行单碱基序列选择性检测。随着向石墨烯器件中添加互补或单碱基错配 DNA,迁移率降低,薄层电阻增加。石墨烯器件的空穴载流子浓度随着互补DNA的加入明显增加,而几乎不受单碱基错配DNA的影响。检测限低至1[公式:见正文]pM,线性范围从1[公式:见正文]pM到100[公式:见正文]nM。此外,霍尔效应生物传感器能够以[公式:见文本]6.2的高特异性将互补DNA与单碱基错配DNA区分开来,这比先前报道的基于DNA的石墨烯生物传感器几乎高出两个数量级。 ——DNA杂交。
更新日期:2020-05-15
中文翻译:
用于提高无标记 DNA 检测的特异性和灵敏度的基于石墨烯的霍尔效应生物传感器
人们广泛关注使用石墨烯或氧化石墨烯 (GO) 片材作为传感器,用于选择性和无标记检测生物分子,例如 DNA、肿瘤标志物、生物离子等。这里,化学气相沉积 (CVD) 石墨烯-报道了一种基于霍尔效应的生物传感器,用于通过 DNA 杂交对 DNA 进行超灵敏的无标记检测。基于 Van der Pauw 方法的霍尔效应测量用于对通过 CVD 制备的石墨烯片上的 DNA 进行单碱基序列选择性检测。随着向石墨烯器件中添加互补或单碱基错配 DNA,迁移率降低,薄层电阻增加。石墨烯器件的空穴载流子浓度随着互补DNA的加入明显增加,而几乎不受单碱基错配DNA的影响。检测限低至1[公式:见正文]pM,线性范围从1[公式:见正文]pM到100[公式:见正文]nM。此外,霍尔效应生物传感器能够以[公式:见文本]6.2的高特异性将互补DNA与单碱基错配DNA区分开来,这比先前报道的基于DNA的石墨烯生物传感器几乎高出两个数量级。 ——DNA杂交。