Multiwalled Carbon Nanotubes (MWCNTs) were used as a support of amperometric enzymatic biosensors of guaiacol. The structural quality of MWCNTs was determined for Raman Spectra and DRX analysis. MWCNTs were decorated with iron oxide nanoparticles (36 w/w%), which were observed by FE-SEM, and were confirmed by with EDX, and TGA analysis. Laccase enzyme (aspergillus sp.) was immobilized on the surface of MWCNTs (oxidized and decorated with iron oxide nanoparticles) confirmed by XPS analysis and used to amperometric detection of guaiacol. The material obtained was deposited on the active surface of glassy carbon electrode (GCE) and was carried out using a typical three-electrode system with saturated calomel electrode as a reference and a graphite rod as a counter-electrode. The results confirm the potential use of bioelectrode Lac/MWCNTs/GCE and Lac/Fe 3 O 4 /MWCNTs/GCE for the guaiacol detection in low concentrations. Amperometric sensitivities and detect...

中文翻译：

---

固定化漆酶的多壁碳纳米管对愈创木酚生物传感器的安培响应研究

多壁碳纳米管（MWCNT）被用作愈创木酚的安培酶生物传感器的支持。通过拉曼光谱和DRX分析确定MWCNT的结构质量。MWCNTs用氧化铁纳米粒子（36 w / w％）修饰，通过FE-SEM观察，并通过EDX和TGA分析确认。漆酶（aspergillus sp。）被固定在MWCNTs的表面（氧化并用氧化铁纳米颗粒修饰），并通过XPS分析确认，并用于安培法测定愈创木酚。将获得的材料沉积在玻璃碳电极（GCE）的活性表面上，并使用典型的三电极系统（饱和甘汞电极作为参比，石墨棒作为对电极）进行处理。结果证实了生物电极Lac / MWCNTs / GCE和Lac / Fe 3 O 4 / MWCNTs / GCE的潜在用途可用于低浓度的愈创木酚检测。安培灵敏度和检测...