Background Epilepsy is a chronic neurological disease. A suitable biomarker for epilepsy diagnosis remains lacking. MicroRNAs (miRNAs) were pronounced as promising biomarkers for epileptogenesis. Objectives To analyze the expression levels of miR 194-5p, miR 106b, and miR 146a in Egyptian epileptic patients compared to control subjects and to detect their correlation to clinical characteristics. Subjects and methods We evaluated the expression levels of miR 106b, miR 146a, and miR 194-5p using real-time quantitative polymerase chain reaction (qRT-PCR) in 50 subjects: 15 patients with idiopathic generalized epilepsy, 15 patients with focal epilepsy (3 idiopathic and 12 cryptogenic), and 20 healthy controls. Results miR 106b and miR 194-5p were upregulated in the generalized epilepsy group compared to control; miR 194-5p was significantly downregulated in the focal epilepsy group compared to the generalized epilepsy group and control ( p ˂ 0.05). miR 194-5p was negatively correlated to disease duration in patients with focal epilepsy; the three microRNAs were positively correlated to each other ( p ˂ 0.05). Conclusion Serum miR 194-5P and miR 106b can be used as potential non-invasive biomarkers in the evaluation of idiopathic generalized epilepsy.

中文翻译：

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循环 miR 194-5p、miR 106b 和 miR 146a 作为癫痫潜在生物标志物的作用：病例对照研究

背景癫痫是一种慢性神经系统疾病。仍然缺乏用于癫痫诊断的合适生物标志物。MicroRNAs (miRNAs) 被认为是癫痫发生的有希望的生物标志物。目的 与对照受试者相比，分析埃及癫痫患者中 miR 194-5p、miR 106b 和 miR 146a 的表达水平，并检测它们与临床特征的相关性。受试者和方法 我们使用实时定量聚合酶链反应 (qRT-PCR) 在 50 名受试者中评估了 miR 106b、miR 146a 和 miR 194-5p 的表达水平：15 名特发性全身性癫痫患者，15 名局灶性癫痫患者（ 3 例特发性和 12 例隐源性）和 20 例健康对照。结果 与对照组相比，全身性癫痫组的 miR 106b 和 miR 194-5p 上调；与全身性癫痫组和对照组相比，局灶性癫痫组的 miR 194-5p 显着下调（ p ˂ 0.05）。miR 194-5p与局灶性癫痫患者的病程呈负相关；三个 microRNA 之间呈正相关（p ˂ 0.05）。结论 血清 miR 194-5P 和 miR 106b 可作为潜在的非侵入性生物标志物用于评估特发性全身性癫痫。