A novel, simple, sensitive and rapid spectrophotometric method for the determination of selenium(IV) in an acidic medium using rhodamine B hydrazide (RBH) has been developed. The method is based on the micellar mediated oxidation of RBH by Se(IV) in an acidic medium to produce a pinkish violet color of rhodamine B, which was monitored spectrophotometrically at λ_max 585 nm. The sensitivity of the method was found to increase when the reaction was performed in a micellar medium. Beer's law was obeyed in the concentration range of 0.002–0.032 μg mL⁻¹. The reaction variables such as time, temperature, reagent concentration, and acidity have been optimized for the reaction. Sandell's sensitivity and molar absorptivity for the reaction system were found to be 0.00004 μg cm⁻² and 42.52 × 10⁶ L mol⁻¹ cm⁻¹, respectively. The developed method was successfully applied for the determination of Se(IV) in several real samples with quantitative results.

中文翻译：

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胶束介导的使用生色试剂测定环境样品中硒的新方法。

开发了一种新颖，简单，灵敏，快速的分光光度法，用于使用罗丹明B酰肼（RBH）测定酸性介质中的硒（IV）。该方法是基于RBH的胶束介导的氧化由硒（IV在产生若丹明B，将其在分光光度法监测的带粉红色的紫色酸性介质中）λ_最大585nm的。当在胶束介质中进行反应时，发现该方法的灵敏度增加。在0.002–0.032μgmL ^-1的浓度范围内遵守比尔定律。反应变量（例如时间，温度，试剂浓度和酸度）已针对该反应进行了优化。发现桑德尔对反应体系的灵敏度和摩尔吸收率分别为0.00004μgcm ^-2和42.52×10 ⁶ L mol ^-1 cm ^-1。所开发的方法已成功地用于测定多个真实样品中的Se（IV），并具有定量结果。