Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health, social, and economic infrastructures. Here, we assess immunogenicity and anamnestic protective efficacy in rhesus macaques of the intradermal (ID)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800. INO-4800 is an ID-delivered DNA vaccine currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and neutralizing antibody responses against both the D614 and G614 SARS-CoV-2 spike proteins. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T and B cell responses. These responses were associated with lower viral loads in the lung and with faster nasal clearance of virus. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system which are likely important for providing durable protection against COVID-19 disease.

中文翻译：

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皮内递送的DNA疫苗可在恒河猴SARS-CoV-2攻击模型中提供记忆消除保护

由SARS-CoV-2病毒引起的2019年冠状病毒病（COVID-19）已对公共卫生，社会和经济基础设施产生了巨大的全球影响。在这里，我们评估了皮内（ID）递送的SARS-CoV-2尖峰DNA疫苗INO-4800在恒河猴中的免疫原性和记忆删除保护功效。INO-4800是ID传递的DNA疫苗，目前正在临床试验中进行评估。用INO-4800疫苗接种可诱导T细胞反应，并中和针对D614和G614 SARS-CoV-2刺突蛋白的抗体反应。疫苗接种后数月，动物受到SARS-CoV-2攻击，从而迅速召回抗SARS-CoV-2突突蛋白T和B细胞反应。这些反应与肺中较低的病毒载量和较快的鼻病毒清除率有关。