Species with genetically differentiated allopatric populations commonly differ in phenotypic traits due to drift and/or selection, which can be important drivers of reproductive isolation. Wedge-tailed sabrewing (Campylopterus curvipennis) is a species complex composed of three genetically and acoustically differentiated allopatric lineages that correspond to currently recognized subspecies in Mexico: C. c. curvipennis (Sierra Madre Oriental), C. c. pampa (Yucatán Peninsula), and C. c. excellens (Los Tuxtlas). Although excellens is taxonomically recognized as a distinct species, there is genetic evidence that lineages excellens and curvipennis have diverged from each other later than pampa. In this study, we experimentally tested C. c. curvipennis song recognition as a major factor in premating reproductive isolation for lineage recognition. To this end, we conducted a song playback experiment to test whether territorial males of one C. c. curvipennis lek discriminate among potential competitors based on male songs from the three lineages. Males of curvipennis responded more aggressively to songs of their own lineage and excellens, than to songs of the most divergent lineage pampa, as evidenced by significant differences in a variety of intensity and latency response variables. This indicate that the pampa male song does not represent a competitive threat as curvipennis and excellens songs, in which divergence and song recognition represent premating reproductive isolation between these isolated lineages. However, the acoustic limits between curvipennis and excellens might be attenuated by gene flow in case of secondary contact between them, despite the strong and relatively rapid divergence of their sexually selected song traits.

中文翻译：

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声音识别表明，过早的蜂鸟谱系之间过早隔离

具有遗传分化异源种群的物种通常由于漂移和/或选择而在表型性状上有所不同，这可能是生殖分离的重要驱动力。楔尾sa（Camylopterus curvipennis）是一个物种复合体，由三种遗传上和声学上不同的异源谱系组成，这些谱系对应于墨西哥目前公认的亚种：C. c。c。curvipennis（东方马德雷山脉），c。潘帕（尤卡坦半岛）和C. c。优秀（Los Tuxtlas）。尽管优秀的动物在分类学上被认为是一个独特的物种，但有遗传证据表明血统优秀和弯曲彼此分开的时间比潘帕晚。在这项研究中，我们通过实验测试了C。弯曲的歌曲识别是提早将生殖隔离用于谱系识别的主要因素。为此，我们进行了歌曲播放实验，以测试一个C. c。的领土男性。curvipennis lek根据来自三个谱系的男性歌曲来区分潜在的竞争对手。雄性curvipennis反应比较积极，以自己的血统和歌曲excellens，比最不同血统的歌曲草原，通过在各种强度和延迟反应变量的显著差异就是证明。这表明潘帕男曲并不代表作为竞争威胁curvipennis和excellens歌曲，其中的分歧和歌曲识别代表premating这些孤立的谱系间生殖隔离。然而，之间的声学限制curvipennis和excellens可能通过它们之间的二次接触的情况下，基因流，尽管他们的性选择的歌曲特质的强劲和相对快速的发散衰减。