Abstract

A facile method to produce iron oxide nanoparticles (FeO NPs) has been introduced as a part of green approach. Here, we introduce a semi-green method to fabricate FeO NPs using Channa straitus (C. straitus). The formation of FeO NPs was optimized with mucus extract (1, 2, and 3 mL) to study the size and morphology. The results reveal that the FeO NPs produced using 2 mL mucus possess the minimum size. The morphology, size, and crystallinity of nanoparticles was confirmed by UV-Vis spectroscopy (UV-Vis), X-ray diffraction (XRD), scanning electron microscopy (SEM), and electron diffraction spectroscopy (EDS). Formation and the functional groups responsible for reduction and stabilization of nanoparticles were confirmed by UV-Vis and FTIR analysis. The synthesized FeO NPs were examined for their size dependent antibacterial activity. The FeO stabilized with mucus exhibited zone of inhibition against Escherichia coli, Streptococcus faecalis, Streptococcus oralis, Bacteroides fragilis, and Proteus vulgaris. The DNA cleavage study revealed that the FeO NPs induce cell death by apoptosis. The results of the present study suggest that the iron oxide might be used for medicinal applications especially for cancer therapeutics.

抽象的

作为绿色方法的一部分，已经引入了一种生产氧化铁纳米颗粒（FeO NPs）的简便方法。在这里，我们介绍一种半绿色方法，使用Channa straitus（C. straitus）制备FeO NP 。）。用粘液提取液（1、2和3 mL）优化了FeO NP的形成，以研究其大小和形态。结果表明，使用2 mL黏液产生的FeO NP具有最小的尺寸。纳米颗粒的形态，大小和结晶度通过紫外可见光谱（UV-Vis），X射线衍射（XRD），扫描电子显微镜（SEM）和电子衍射光谱（EDS）得以确认。通过UV-Vis和FTIR分析确认了负责纳米颗粒还原和稳定的形成和官能团。检查合成的FeO NP的大小依赖性抗菌活性。用粘液稳定的FeO表现出对大肠杆菌，粪链球菌，口腔链球菌，脆弱拟杆菌和寻常变形杆菌。DNA裂解研究表明，FeO NPs通过凋亡诱导细胞死亡。本研究的结果表明，氧化铁可用于医学应用，尤其是用于癌症治疗。