Tigecycline is a relatively new antimicrobial, belonging to glycylcyclines with antimicrobial activity against a large spectrum of bacteria. Very few data are available on its effect on Leptospira spp., which consist in a bacteriostatic mechanism. The aim of this investigation was to evaluate the bacteriostatic and bactericidal effect of tigecycline on reference Leptospira strains belonging to 16 serovars. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined through the microdilutions method, and tetracycline was used as the control. Results showed that tigecycline had higher MIC and MBC values than tetracycline. Obtained MIC values were between 4 and 32 µg/mL, while MBC values between 16 and >128 µg/mL. Patoc (MIC: 4 µg/mL; MBC: 16 µg/mL) resulted in the most susceptible serovar, while the most resistant were Bataviae (MIC: 32 µg/mL; MBC: 64 µg/mL), Bratislava (MIC: 8 µg/mL; MBC 128 µg/mL), and Tarassovi (MIC: 8 µg/mL; MBC: >128 µg/mL). This is the first investigation focused on the effect of tigecycline against Leptospira spp. reference strains. Since tigecycline is used as a treatment for bacteremia and urinary tract disease, and these symptoms could be linked to Leptospira infection, the possibility of using this antibiotic as a treatment for leptospirosis should be evaluated. Further studies are needed to explore the possibility to use tigecycline for in vivo application against Leptospira.

中文翻译：

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替加环素对钩端螺旋体的抑菌和杀菌作用。

替加环素是一种相对较新的抗菌剂，属于糖基环素，对多种细菌均具有抗菌活性。关于它对钩端螺旋体的影响的很少数据，这是一种抑菌机制。这项研究的目的是评估替加环素对参考钩端螺旋体的抑菌和杀菌作用。属于16个血清型的菌株。通过微量稀释法确定最小抑菌浓度（MIC）和最小杀菌浓度（MBC），并以四环素为对照。结果表明，替加环素的MIC和MBC值高于四环素。获得的MIC值介于4和32 µg / mL之间，而MBC值介于16和> 128 µg / mL之间。Patoc（MIC：4 µg / mL; MBC：16 µg / mL）导致最易感的血清型，而耐药性最高的是Bataviae（MIC：32 µg / mL; MBC：64 µg / mL），布拉迪斯拉发（MIC：8 µg / mL； MBC 128 µg / mL）和Tarassovi（MIC：8 µg / mL； MBC：> 128 µg / mL）。这是第一个针对替加环素抗钩端螺旋体作用的研究spp。参考菌株。由于替加环素被用作菌血症和尿道疾病的治疗方法，并且这些症状可能与钩端螺旋体感染有关，因此应评估使用这种抗生素治疗钩端螺旋体病的可能性。需要进一步的研究来探索可能使用替加环素在体内 对应用钩端螺旋体。