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Trehalose and (iso)floridoside production under desiccation stress in red alga Porphyra umbilicalis and the genes involved in their synthesis
Journal of Phycology ( IF 2.9 ) Pub Date : 2020-07-29 , DOI: 10.1111/jpy.13057
Yuanyu Cao 1 , David J. Ashline 2 , Elizabeth Ficko‐Blean 3 , Anita S. Klein 4
Affiliation  

The marine red alga Porphyra umbilicalis has high tolerance toward various abiotic stresses. In this study, the contents of floridoside, isofloridoside, and trehalose were measured using gas chromatography mass spectrometry (GC‐MS) in response to desiccation and rehydration treatments; these conditions are similar to the tidal cycles that P. umbilicalis experiences in its natural habitats. The GC‐MS analysis showed that the concentration of floridoside and isofloridoside did not change in response to desiccation as expected of compatible solutes. Genes involved in the synthesis of (iso)floridoside and trehalose were identified from the recently completed Porphyra genome, including four putative trehalose‐6‐phosphate synthase (TPS) genes, two putative trehalose‐6‐phosphate phosphatase (TPP) genes, and one putative trehalose synthase/amylase (TreS) gene. Based on the phylogenetic, conserved domain, and gene expression analyses, it is suggested that the Pum4785 and Pum5014 genes are related to floridoside and isofloridoside synthesis, respectively, and that the Pum4637 gene is probably involved in trehalose synthesis. Our study verifies the occurrences of nanomolar concentrations trehalose in P. umbilicalis for the first time and identifies additional genes possibly encoding trehalose phosphate synthases.

中文翻译:

干旱胁迫下红藻紫菜中海藻糖和(异)氟尿苷的产生及其合成基因

海洋红藻Popphyra umbilicalis对各种非生物胁迫具有很高的耐受性。在这项研究中,响应于干燥和补液处理,使用气相色谱质谱法(GC-MS)测定了花色苷,异花苷和海藻糖的含量。这些条件类似于脐带假单胞菌在其自然栖息地中经历的潮汐周期。GC-MS分析表明,与干燥溶质反应所期望的干燥程度相比,花色苷和异花苷的浓度没有变化。从最近完成的紫菜中鉴定了与(异)氟尿苷和海藻糖合成有关的基因基因组,包括四个推定的海藻糖-6-磷酸合酶(TPS)基因,两个推定的海藻糖-6-磷酸磷酸酶(TPP)基因和一个推定的海藻糖合酶/淀粉酶(TreS)基因。根据系统发育,保守结构域和基因表达分析,表明Pum4785Pum5014基因分别与floridoside和isofloridoside合成有关,并且Pum4637基因可能参与海藻糖合成。我们的研究首次验证了脐橙假单胞菌中纳摩尔浓度海藻糖的出现,并鉴定了可能编码海藻糖磷酸合酶的其他基因。
更新日期:2020-07-29
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