Cannabinoid receptor type 1 (CB1R) modulates synaptic activity and is widely distributed in brain areas such as the hippocampus, cerebellum, cerebral cortex, and striatum, among others. CB1R is involved in processes such as memory, learning, motor coordination, and mood. Genetic deletion of CB1R causes behavioral alterations. In this work, we evaluated neuronal morphology and synaptic structure in the hippocampus of adult male CB1R knockout mice (CB1R^−/−). Morphological changes in the CB1R^−/− hippocampus evidenced a decrease in the expression of cytoskeletal proteins neurofilaments 160 KDa, neurofilaments 200 KDa, and microtubule‐associated protein 2. CA1 neurons showed decreased arborization and changes in synaptic structure such as lower thickness of postsynaptic density and a reduction in synaptophysin levels. Results obtained in the present work provide evidence of the participation of CB1R in the establishment of neuronal structure and networks that could have an important role in neuronal plasticity. In addition, these changes observed in CB1R^−/− could be correlated with behavioral alterations reported.

中文翻译：

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大麻素受体 1 型基因敲除小鼠海马的神经元和突触形态学改变。

大麻素受体 1 (CB1R) 调节突触活动，广泛分布于大脑区域，如海马、小脑、大脑皮层和纹状体等。CB1R 参与记忆、学习、运动协调和情绪等过程。CB1R 的基因缺失导致行为改变。在这项工作中，我们评估了成年雄性 CB1R 敲除小鼠 (CB1R ^-/- )海马中的神经元形态和突触结构。CB1R 的形态变化^-/-海马体证明细胞骨架蛋白神经丝 160 KDa、神经丝 200 KDa 和微管相关蛋白 2 的表达减少。 CA1 神经元显示出树枝化减少和突触结构变化，例如突触后密度降低和突触素水平降低。在目前的工作中获得的结果提供了 CB1R 参与建立神经元结构和网络的证据，这些结构和网络可能在神经元可塑性中起重要作用。此外，在 CB1R ^{-/- 中}观察到的这些变化可能与报告的行为改变相关。