Pharmaceuticals are widespread in aquatic ecosystem, which may pose a potential threat to fish and human. In the study, a robust and reliable magnetic solid-phase extraction (MSPE) based on bovine serum albumin (BSA) restricted access octadecyl/phenyl-mixed-functionalized magnetic silica nanoparticles (BSA-C18/Ph-Fe₃O₄@SiO₂ NPs) as sorbent was developed for the extraction of venlafaxine, paroxetine, fluoxetine, norfluoxetine, sertraline and diphenhydramine from the muscle extracts of aquatic products followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) detection. The homemade sorbent showed appropriate compatibility in aqueous solution and good performance in reducing matrix interference of snakehead muscle tissue extracts with absolute matrix effect ranging in 95.4%–105.5%. The protocol was validated in analyte-free snakehead muscle with favorable recoveries ranging in 91.6%–103.6% and relative standard deviations (RSDs) less than 9.5%. Limits of detection (LODs) of the drugs were lower than 0.018 μg kg^–1. Short-term drug-exposure experiments at low and high doses were conducted on snakeheads, and the measured contents of analytes were in the range 0.029–9.58 μg kg^–1 with appropriate recoveries (90.0%–114.0%). The approach was extensively applied for the analysis of twelve species of market-sale aquatic products (total 37 samples), and up to 1.868 and 0.521 μg kg^–1 of diphenhydramine and venlafaxine were measured, respectively. The approach shows remarkable potential in biological complex samples.

药物广泛存在于水生生态系统中，可能对鱼类和人类构成潜在威胁。在这项研究中，基于牛血清白蛋白 (BSA) 的稳健可靠的磁性固相萃取 (MSPE) 限制了十八烷基/苯基混合功能化磁性二氧化硅纳米粒子 (BSA-C18/Ph-Fe ₃ O ₄ @SiO ₂NPs) 作为吸附剂开发用于从水产品的肌肉提取物中提取文拉法辛、帕罗西汀、氟西汀、去甲氟西汀、舍曲林和苯海拉明，然后进行超高效液相色谱-串联质谱 (UHPLC-MS/MS) 检测。自制吸附剂在水溶液中表现出适当的相容性，在降低黑鱼肌肉组织提取物的基质干扰方面表现良好，绝对基质效应在95.4%~105.5%之间。该方案在无分析物的蛇头肌肉中得到验证，良好的回收率范围为 91.6%–103.6%，相对标准偏差 (RSD) 小于 9.5%。药物的检测限 (LOD) 低于 0.018 μg kg ^–1. 对蛇头进行了低剂量和高剂量的短期药物暴露实验，测定的分析物含量在 0.029-9.58 μg kg ^-1范围内，回收率适当（90.0%-114.0%）。该方法被广泛应用于12种市售水产品（共37个样品）的分析，分别测得苯海拉明和文拉法辛分别高达1.868和0.521 μg kg ^{-1 。}该方法在生物复杂样品中显示出显着的潜力。