Three strains of rhizobia isolated from effective root nodules of pea (Pisum sativum L.) collected from the Indian trans-Himalayas were characterized using 16S rRNA, atpD and recA genes. Phylogeny of the 16S rRNA genes revealed that the newly isolated strains were members of the genus Rhizobium with ≥99.9% sequence similarity to the members within the “Rhizobium leguminosarum” group. Phylogenetic analyses based on the concatenated sequences of atpD and recA gene, and 92 core genes extracted from the genome sequences indicated that strains JKLM 12A2^T and JKLM 13E are grouped as a separate clade closely related to R. laguerreae FB206^T. In contrast, the strain JKLM 19E was placed with “R. hidalgonense” FH14^T. Whole-genome average nucleotide identity (ANI) values were 97.6% within strains JKLM 12A2^T and JKLM 13E, and less than 94% with closely related species. The digital DNA-DNA hybridization (dDDH) values were 81.45 within the two strains and less than 54.8% to closely related species. The major cellular fatty acids were C_18:1w7c in summed feature 8, C_{14:0 3OH}/C_{16:1 iso I} in summed feature 2, and C_18:0. The DNA G + C content of JKLM 12A2^T and JKLM 13E was 60.8 mol%. The data on genomic, chemotaxonomic, and phenotypic characteristics indicates that the strains JKLM 12A2^T and JKLM 13E represent a novel species, Rhizobium indicum sp. nov. The type strain is JKLM 12A2^T (= MCC 3961^T = KACC 21380^T = JCM 33658^T). However, the strain JKLM 19E represents a member of “R. hidalgonense” and the symbiovar viciae.

使用16S rRNA，atpD和recA基因鉴定了从印度反喜马拉雅州收集的从豌豆（Pisum sativum L.）的有效根瘤中分离出的三株根瘤菌。16S rRNA基因的系统发育分析表明，新分离出的菌株是根瘤菌属的成员，与“根瘤菌”组中的成员具有≥99.9％的序列相似性。基于atpD和recA基因的串联序列以及从基因组序列中提取的92个核心基因的系统发育分析表明，菌株JKLM 12A2 ^T和JKLM 13E被分组为与R密切相关的单独进化枝。laguerreae FB206^牛逼。相反，将菌株JKLM 19E放置为“ R”。hidalgonense ” FH14^牛逼。JKLM 12A2 ^T和JKLM 13E菌株的全基因组平均核苷酸同一性（ANI）值为97.6％，而密切相关的物种则不到94％。在两个菌株中，数字DNA-DNA杂交（dDDH）值为81.45，与紧密相关的物种相比，小于54.8％。主要的细胞脂肪酸是特征8的C _18：1w7c，特征2 2的C _{14：0 3OH} / C _{16：1 iso I}和特征C _18：0。JKLM 12A2 ^T的DNA G + C含量JKLM 13E为60.8摩尔％。基因组，化学分类学和表型特征的数据表明，菌株JKLM 12A2 ^T和JKLM 13E代表了一种新种，印度根瘤菌。十一月 类型应变为JKLM 12A2 ^T（= MCC 3961 ^T  = KACC 21380 ^T  = JCM 33658 ^T）。但是，菌株JKLM 19E代表“ R ”的成员。hidalgonense ”和共生菌蚕豆。