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Combined effect of salinity and pH on lipid content and fatty acid composition of Tisochrysis lutea
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2020-07-30 , DOI: 10.1016/j.sjbs.2020.07.027
Adel W. Almutairi , Abo El-Khair B. El-Sayed , Marwa M. Reda

The haptophyte microalga Tisochrysis lutea was heterotrophically grown in F2 medium with different combinations of pH and salinity. Growth, oil content and fatty acids (FAs) profile were determined under each set of conditions. The salinity was adjusted using NaCl at concentrations of 0.4, 0.6, 0.8, or 1.0 M, while pH was adjusted at 7, 8, or 9, and heterotrophic growth was performed using organic carbon in the form of sugar cane industry waste (CM). Fatty acid methyl esters (FAMEs) were identified by gas chromatography. The results showed that pH of 8.0 was the optimal for dry weight and oil production, regardless of the salinity level. At pH 8.0, growth at a salinity of 0.4 M NaCl was optimal for biomass accumulation (1.185 g L-1). Under these conditions, the maximum growth rate was 0.055 g L-1 d-1, with a doubling time of 17.5 h and a degree of multiplication of 2.198. Oil content was maximal (34.87%) when the salinity was 0.4 M and the pH was 9.0. The ratio of saturated to unsaturated FAs was affected by the pH value and salinity, in that unsaturated FAs increased to 58.09% of the total FAs, considerably greater than the value of 40.59% obtained for the control (0.4 M NaCl and pH 8.0).



中文翻译:

盐度和pH值联合作用对黄褐毛线虫脂质含量和脂肪酸组成的影响

将触藻微藻黄褐线虫在pH和盐度不同的F2培养基中异养生长。在每组条件下确定生长,油含量和脂肪酸(FA)分布。使用浓度为0.4、0.6、0.8或1.0 M的NaCl调节盐度,同时将pH调节为7、8或9的pH,并使用甘蔗工业废料(CM)形式的有机碳进行异养生长。脂肪酸甲酯(FAME)通过气相色谱法鉴定。结果表明,无论盐度水平如何,pH 8.0都是干重和产油量的最佳选择。在pH 8.0时,盐度为0.4 M NaCl的生长最适合生物质积累(1.185 g L -1)。在这些条件下,最大生长速率为0.055 g L -1 d -1,倍增时间为17.5 h,繁殖度为2.198。当盐度为0.4 M,pH为9.0时,含油量最大(34.87%)。饱和脂肪酸与不饱和脂肪酸的比率受pH值和盐度的影响,因为不饱和脂肪酸增加至总脂肪酸的58.09%,大大高于对照(0.4 M NaCl和pH 8.0)获得的40.59%。

更新日期:2020-07-30
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