The nuclear factor-κB (NF-κB) family is evolutionary conserved and plays key roles in the regulation of numerous basic cellular processes. In this study, a sea cucumber Holothuria leucospilota NF-κB1 p105 named HLp105 was first obtained. The full-length cDNA of HLp105 is 6564 bp long, with a 219 bp 5′ untranslated region (UTR), a 2979 bp 3′ UTR, and a 3366 bp open reading frame (ORF) encoding for 1121 amino acids with a deduced molecular weight of 123.92 kDa and an estimated pI of 5.31. HLp105 protein contains the conserved domain RHD, IPT, ANK and DEATH. HLp105 mRNA can be detected in all tissues examined, with the highest level in the intestine, followed by the transverse vessel, rete mirabile, coelomocytes, respiratory tree, bolishiti, cuvierian tubules, body wall, oesophagus and muscle. Challenged by LPS or poly (I:C), the transcription level of HLp105 was apparently up-regulated in the tissues examined. Besides, Over-expression of HLp105 in HEK293T cells, the apoptosis was inhibited, and the cytokines IL-1β and TNF-α were activated. The results are important for better understanding the function of NF-κB1 p105 in sea cucumber and reveal its involvement in immunoreaction.

核因子-κB (NF-κB) 家族在进化上是保守的，在许多基本细胞过程的调节中起关键作用。在这项研究中，一种海参Holothuria leucospilota首次获得命名为 HLp105 的 NF-κB1 p105。HLp105 的全长 cDNA 长 6564 bp，具有 219 bp 5' 非翻译区 (UTR)、2979 bp 3' UTR 和 3366 bp 开放阅读框 (ORF)，编码 1121 个氨基酸，推导分子重量为 123.92 kDa，估计 pI 为 5.31。HLp105 蛋白包含保守结构域 RHD、IPT、ANK 和 DEATH。HLp105 mRNA可在所有检查的组织中检测到，在肠道中水平最高，其次是横管、奇异网、体腔细胞、呼吸树、bolishiti、居维叶小管、体壁、食道和肌肉。受到 LPS 或 poly (I:C) 的挑战，HLp105 的转录水平在检查的组织中明显上调。此外，在 HEK293T 细胞中过表达 HLp105，细胞凋亡受到抑制，细胞因子IL-1β和TNF-α被激活。该结果对于更好地了解海参中NF-κB1 p105的功能并揭示其参与免疫反应具有重要意义。