Abstract The dried blood spot (DBS) is widely used for collecting microvolume blood for examining free amino acid concentrations in the blood for patients with defects in amino acid metabolism. However, comparing to many clinical tests which are based on cell-free plasma, the DBS system collects whole blood, where the presence of red blood cells (RBCs) may influence the qualitative and quantitative data analyses. Thus, we report novel membranes for decellularization of blood in the dried plasma spot (DPS) system. The membrane was held tightly with an absorbent paper underneath for collecting the permeated cell-free plasma. By combining the thermally induced phase separation (TIPS) and non-solvent induced phase separation (NIPS), i.e., N-TIPS, we have developed flat-sheet PAN membranes not only able to remove almost 100% RBCs but also allow most plasma to permeate through them effectively. The optimized membrane also has almost no retention to a few targeted amino acids.

中文翻译：

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由非溶剂热致相分离 (N-TIPS) 制成的膜，用于干血浆点 (DPS) 应用中的血液脱细胞

摘要 干血斑（DBS）广泛用于采集微量血液，用于检测氨基酸代谢缺陷患者血液中游离氨基酸的浓度。然而，与许多基于无细胞血浆的临床测试相比，DBS 系统收集全血，其中红细胞 (RBC) 的存在可能会影响定性和定量数据分析。因此，我们报告了用于干血浆点 (DPS) 系统中血液脱细胞的新型膜。膜下面用吸水纸紧紧固定，用于收集渗透的无细胞血浆。通过结合热致相分离 (TIPS) 和非溶剂致相分离 (NIPS)，即 N-TIPS，我们开发了平板 PAN 膜，不仅能够去除几乎 100% 的红细胞，而且还能让大多数血浆有效地渗透通过它们。优化后的膜对少数目标氨基酸几乎没有保留。