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A galactose-rich heteropolysaccharide extracted from "jaboticaba" (Plinia cauliflora) peels.
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2020-07-30 , DOI: 10.1016/j.carbpol.2020.116821
Bruna M Miranda 1 , Maria C B Di-Medeiros 2 , Karla A Batista 3 , Elaine R Carbonero 4 , Kátia F Fernandes 5 , Flávio A Silva 6
Affiliation  

The objective of this work was to extract, identify and characterize a galactose-rich heteropolysaccharide (GH) from “jaboticaba” peel. The best conditions to extract the GH according to a 23 full-factorial experimental design were 90 °C/30 min/pH 1.0, resulting in a 32.32 % yield using lyophilized sample. The chemical structure analyzed by GC/MS and NMR spectra (HSQC/HSQC-TOCSY) showed that the main chain of GH consists of a (1→4) galactoside branched at carbon 3, containing galactose (67.21 %), glucose (15.78 %), arabinose (9.78 %), rhamnose (2.26 %) and traces of esterified and non-esterified uronic acids. Rheological studies revealed that GH suspensions behave as a Newtonian fluid, with calculated molecular mass of 1.48 × 105 Da. The absolute viscosity of 1 % (w/v) aqueous suspension of GH decreased from 25 mPa s to 10 mPa s in NaCl and 7 mPa s in CaCl2, indicating the polyelectrolyte character of GH.



中文翻译:

从“ jaboticaba”(Plinia cauliflora)果皮中提取的富含半乳糖的杂多糖。

这项工作的目的是从“ jaboticaba”果皮中提取,鉴定和鉴定富含半乳糖的杂多糖(GH)。根据2 3全因子实验设计,提取GH的最佳条件是90°C / 30 min / pH 1.0,使用冻干样品可获得32.32%的收率。通过GC / MS和NMR光谱(HSQC / HSQC-TOCSY)分析的化学结构表明,GH的主链由(1→4)在碳3上分支的半乳糖苷组成,含半乳糖(67.21%),葡萄糖(15.78%) ),阿拉伯糖(9.78%),鼠李糖(2.26%)和痕量的酯化和未酯化的糖醛酸。流变学研究表明,GH悬浮液表现为牛顿流体,计算分子量为1.48×10 5大。GH的1%(w / v)水悬浮液的绝对粘度在NaCl中从25 mPa s降低到10 mPa s,在CaCl 2中从7 mPa s降低,表明GH的聚电解质特性。

更新日期:2020-08-12
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