Biosynthesis is a promising green method to produce hydrogen fuel. Different approaches have been used in this regard to enhance hydrogen yield, however, there is still a need to explore further. The current research is an effort to enhance hydrogen yield using nanoscale organic-iron (NO–Fe) complex. NO–Fe complex was synthesized using Eucalyptus viminalis leaf extract and was characterized by UV–Vis, FTIR, XRD, FESEM, TEM and EDS. The NO–Fe complex was used as an iron source for the newly isolated photo fermentative Rhodopseudomonas palustris MP2 and Rhodopseudomonas palustris MP4 bacterial strains. FeSO₄·7H₂O was also used as a source of iron and compared with the effects of NO–Fe complex. Both the strains responded well to the NO–Fe complex and production of hydrogen was increased with increasing concentrations of NO–Fe complex. Highest yield of hydrogen was achieved by R. palustris MP4 (0.08 ± 0.01 mol-H₂mol⁻¹ of-succinate) when the NO–Fe complex was used at 5.0 mg L⁻¹ concentration. The NO–Fe complex was more efficient than iron ions in enhancing bio-hydrogen production. The study revealed that phyto-fabricated NO–Fe complex is an efficient nutrient to improve hydrogen production by purple non-sulfur bacteria.

生物合成是一种有前途的绿色生产氢燃料的方法。在这方面已经使用了不同的方法来提高氢产率，但是，仍然需要进一步探索。当前的研究是使用纳米级有机铁（NO–Fe）络合物提高氢产率的努力。NO-Fe络合物是用紫桉叶提取物合成的，并用UV-Vis，FTIR，XRD，FESEM，TEM和EDS表征。NO-Fe复合物被用作新分离的光发酵红假单胞菌MP2和红假单胞菌MP4细菌菌株的铁源。FeSO ₄ ·7H ₂O还被用作铁的来源，并与NO-Fe络合物的作用进行了比较。两种菌株对NO-Fe络合物的反应都很好，并且随着NO-Fe络合物浓度的增加，氢的产生也增加了。当使用浓度为5.0 mg L ^-1的NO-Fe络合物时，通过palustris R. MP4（0.08±0.01 mol-H ₂ mol ^-1的琥珀酸酯）可获得最高的氢气产率。NO-Fe络合物在增强生物氢产生方面比铁离子更有效。研究表明，用植物制造的NO-Fe复合物是一种有效的养分，可提高紫色非硫细菌的产氢量。