Lymph node metastasis (LNM) is a critical cause for disease progression and treatment failure in cervical cancer. However, the mechanism underlying cervical cancer LNM remains unclear. In this study, HN1 was found to be dramatically upregulated in cervical cancer and patients with higher HN1 expression are more likely to exhibit a higher rate of LNM and lower survival rate. Univariate and multivariate Cox-regression analyses showed that HN1 is an independent prognostic factor in cervical cancer. Meanwhile, HN1 promotes lymphangiogenesis of cervical cancer in vitro. The in vivo experiment also indicates that HN1 enhances LNM in cervical cancer. Furthermore, we also found that HN1 activated the NF-κB signaling pathway to enhance the expression of downstream genes. Taken together, our study suggests that HN1 plays a crucial role in promoting LNM and acts as a prognostic biomarker in cervical cancer.

淋巴结转移（LNM）是导致宫颈癌疾病进展和治疗失败的重要原因。但是，宫颈癌LNM的潜在机制仍不清楚。在这项研究中，发现HN1在宫颈癌中显着上调，具有较高HN1表达的患者更有可能表现出较高的LNM发生率和较低的生存率。单因素和多因素Cox回归分析显示HN1是宫颈癌的独立预后因素。同时，HN1在体外促进子宫颈癌的淋巴管生成。在体内实验还表明，HN1增强宫颈癌中的LNM。此外，我们还发现HN1激活NF-κB信号通路来增强下游基因的表达。综上所述，我们的研究表明HN1在促进LNM中起着至关重要的作用，并作为宫颈癌的预后生物标志物。