Abstract Anthocyanins extracted from black-carrot were encapsulated into halloysite nanotubes (HNT) as a potential carrier for the first time. Anthocyanins were loaded into HNT under vacuum conditions. TEM images indicated the successful entrance of anthocyanins into the lumen of HNT. Anthocyanins loading efficiency was determined to be 4% using thermogravimetric analysis (TGA). XRD patterns showed that all reflections shifted to higher values for HNT loaded with anthocyanins compared to pure HNT which indicates reduced d-value of the crystalline forms. In vitro release of anthocyanins was studied under gastrointestinal conditions using the pH-differential method. The drug release from HNT was modeled and based on R2 values, the Korsmeyer-Peppas model was introduced as the best model predicting the release behavior. Cytotoxic effects of the samples against MCF-7 human breast cancer and HT-29 colon cancer cell lines were evaluated using SRB assay. The cell viability was reduced by 2 folds against anthocyanins-loaded HNT compared to pure anthocyanins in both cell lines. Anthocyanins-loaded HNT is suggested as a promising pH-responsive drug delivery system for the treatment of cancer.

中文翻译：

---

设计一种 pH 响应药物递送系统，用于释放装载在埃洛石纳米管中的黑胡萝卜花青素，用于癌症治疗

摘要 从黑胡萝卜中提取的花青素首次被封装到埃洛石纳米管（HNT）中作为潜在的载体。在真空条件下将花青素加载到 HNT 中。TEM 图像表明花青素成功进入 HNT 管腔。使用热重分析 (TGA) 确定花青素负载效率为 4%。XRD 图案显示，与纯 HNT 相比，负载花青素的 HNT 的所有反射都转移到更高的值，这表明结晶形式的 d 值降低。在胃肠条件下使用 pH 差异方法研究了花青素的体外释放。对 HNT 的药物释放进行建模，并基于 R2 值，引入 Korsmeyer-Peppas 模型作为预测释放行为的最佳模型。使用 SRB 测定评估样品对 MCF-7 人乳腺癌和 HT-29 结肠癌细胞系的细胞毒性作用。在两种细胞系中，与纯花青素相比，负载花青素的 HNT 的细胞活力降低了 2 倍。负载花青素的 HNT 被认为是一种很有前途的 pH 响应药物递送系统，用于治疗癌症。