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Effects of N-carbamylglutamate on steroidogenesis and relative abundances of mRNA transcripts in pig placental trophoblasts.
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-07-30 , DOI: 10.1016/j.anireprosci.2020.106569
Shangli Wei 1 , Yuze Yang 2 , Yinxia Xiao 1 , Yan Liu 1 , Jianhui Tian 3 , Leon J Spicer 4 , Tao Feng 1
Affiliation  

Supplementation of N-carbamylglutamate (NCG) improves gestation outcomes, with increased piglet within-litter uniformity of birth weight and reduced peripheral steroid concentrations in pregnant sows and ewes. It was hypothesized that the effect of NCG on placental function results from direct effects on the placental trophoblasts. There, therefore, was investigation of the effects of NCG on pig placental trophoblast (pTr) steroidogenesis, mRNA transcript abundance, and cell proliferation in vitro. The pTr were treated with NCG in serum-free medium for 24–48 h. Treatment with NCG inhibited pTr progesterone, androstenedione, testosterone (all P < 0.01), and estradiol (P < 0.05) production, whereas it promoted (P < 0.05) pTr proliferation. Treatment with NCG suppressed (P < 0.05) the relative abundances of CYP11A1, CYP19A1, and CASP3 and increased abundances of CCDN1 (P < 0.01) and CDK4 (P < 0.05) mRNA transcripts in pTr, whereas NCG treatment had no effect (P > 0.10) on relative abundances of StAR, HSD17B4, or HSD3B mRNA transcripts. Treatments with NCG can increase pTr cell numbers of sows through upregulating CCND1 and CDK4 and suppressing CASP3 mRNA transcript abundances, while modulating steroidogenesis through effects on CYP11A1 and CYP19A1 mRNA transcript abundances. It is concluded that NCG may have a direct action on pTr and may regulate placental function by suppressing pTr differentiation as a consequence of lesser steroid synthesis while promoting pTr proliferation and inhibiting apoptosis in sows.



中文翻译:

N-氨基甲酰谷氨酸对猪胎盘滋养细胞中类固醇生成和mRNA转录物相对丰度的影响。

补充N-氨基甲酰谷氨酸 (NCG) 可改善妊娠结局,增加仔猪出生体重的窝内均匀性,并降低怀孕母猪和母羊的外周类固醇浓度。假设 NCG 对胎盘功能的影响源于对胎盘滋养细胞的直接影响。因此,研究了 NCG 对猪胎盘滋养层 (pTr) 类固醇生成、mRNA 转录丰度和体外细胞增殖的影响。pTr 在无血清培养基中用 NCG 处理 24-48 小时。NCG 治疗抑制了 pTr 孕酮、雄烯二酮、睾酮(均P  < 0.01)和雌二醇(P  < 0.05)的产生,而它促进了(P < 0.05) pTr 增殖。治疗NCG抑制(P  <0.05)的相对丰度CYP11A1CYP19A1,和CASP3和增加的丰度的CCDN1P  <0.01)和CDK4P  <0.05)PTR mRNA转录物,而NCG治疗没有效果(P  > 0.10) 关于StARHSD17B4HSD3B mRNA 转录本的相对丰度。NCG治疗可以通过上调CCND1CDK4并抑制CASP3来增加母猪的pTr细胞数量mRNA 转录本丰度,同时通过影响CYP11A1CYP19A1 mRNA 转录本丰度来调节类固醇生成。得出的结论是,NCG 可能对 pTr 有直接作用,并可能通过抑制 pTr 分化(作为较少的类固醇合成的结果)来调节胎盘功能,同时促进 pTr 增殖并抑制母猪的细胞凋亡。

更新日期:2020-08-01
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