Radix Astragali is a traditional Chinese medicine (TCM), which strengthens the efficacy in tonifying Qi after honey-processing. However, a comparative study on the marker compounds in raw and honey-processed Radix Astragali has not been reported. A rapid and sensitive high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of astragaloside IV and calycosin-7-O-β-glucoside from raw and honey-processed Radix Astragali. The method employed silica gel G F₂₅₄ thin-layer plates as the stationary phase, with chloroform–methanol–water (13:7:2 v/v) as the mobile phase. Following development, the plates were observed under ultraviolet (UV) light at 365 nm. The method was validated for linearity, precision, recovery, limit of detection (LOD), and limit of quantification (LOQ). Recovery of the two compounds was between 99.10% and 99.74%. LOD and LOQ were in the range of 15–42 ng and 45–126 ng for the two compounds, respectively. The method was successfully utilized to analyze the two marker components in raw and honey-processed Radix Astragali. The quantitative results demonstrated that astragaloside IV and calycosin-7-glucoside could be converted into their aglycones in the honey-processing procedure. This established HPTLC method would be sensitive enough and reliable for the comprehensive quality control of raw and processed Radix Astragali.

中文翻译：

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高效薄层色谱法同时定量生和蜜加工的黄芪中的两种活性成分

黄芪是一种中药（TCM），可增强蜂蜜加工后补气的功效。但是，尚未对生的和蜂蜜加工的黄芪中的标记物进行比较研究。建立了一种快速灵敏的高效薄层色谱法（HPTLC），用于分析生和蜜加工的黄芪中的黄芪甲苷IV和calycosin-7-O-β-葡萄糖苷。该方法采用硅胶GF ₂₅₄薄层板作为固定相，氯仿-甲醇-水（13：7：2 v / v）作为流动相。显影后，在365nm的紫外线（UV）下观察板。验证了该方法的线性，精密度，回收率，检测限（LOD）和定量限（LOQ）。两种化合物的回收率在99.10％至99.74％之间。这两种化合物的LOD和LOQ分别在15-42 ng和45-126 ng范围内。该方法已成功地用于分析生和蜜加工的黄芪中的两种标记物成分。定量结果表明，在蜂蜜加工过程中，黄芪甲苷IV和calycosin-7-葡萄糖苷可以转化为其糖苷配基。建立的HPTLC方法对于灵芝和黄芪的综合质量控制将足够灵敏和可靠。