Herbal medicine is gaining its popularity day by day and gradually streaming toward integration into the mainstream healthcare systems. Therefore, standardization of herbal formulations is essential in order to assess quality of the drugs. Two commonly used paradigms, compound and pattern-based approaches, were performed to ensure quality and hence product consistency. A rapid, sensitive, and reproducible high-performance thin-layer chromatography (HPTLC) method has been developed for one of the major bioactive markers, piperine. The plates were developed using the mobile phase with toluene–ethyl acetate–formic acid–methanol (3:3:0.8:0.2, v/v). The method was validated for limit of detection, limit of quantification, linearity, specificity, precession, and recovery. The results indicate that the bioactive compound was present in 2.64% w/w in the fruit of Piper nigrum Linn. and 0.253% in a herbal formulation. Each ingredient was analyzed vis-a-vis the herbal formulation and the reflectance spectrum of the matching component(s) to ensure the presence of each ingredient in the herbal formulation. The individual component adding up has also resulted similar fingerprint pattern as that of herbal formulation. Thus, the TLC method could be a rapid tool for the delineation of each individual ingredient in the finished products for authenticity, efficacy, and for batch-to-batch consistency.

中文翻译：

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TLC在确保草药配方中各个成分表现出的作用

草药日益普及，并逐渐融入主流医疗保健系统。因此，为了评估药物质量，草药配方的标准化至关重要。进行了两种常用的范例，即基于复合方法和基于模式的方法，以确保质量并因此确保产品的一致性。已经开发出一种快速，灵敏且可重现的高性能薄层色谱（HPTLC）方法，用于一种主要的生物活性标记物胡椒碱。板使用甲苯-乙酸​​乙酯-甲酸-甲醇（3：3：0.8：0.2，v / v）。验证了该方法的检出限，定量限，线性，特异性，进动和回收率。结果表明，在Piper nigrum Linn的果实中生物活性化合物的含量为2.64％w / w。在草药配方中为0.253％。针对草药制剂和匹配组分的反射光谱对每种成分进行分析，以确保草药制剂中每种成分的存在。各个成分加起来也产生了与草药配方相似的指纹图谱。因此，TLC方法可能是快速鉴定最终产品中每种成分的真实性，功效和批次间一致性的工具。