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Clinical and histopathological changes in rainbow trout Oncorhynchus mykiss experimentally infected with fungus Bradymyces oncorhynchi.
Folia Microbiologica ( IF 2.6 ) Pub Date : 2020-07-29 , DOI: 10.1007/s12223-020-00813-5
J Řehulka 1 , M Kolařík 2, 3 , V Hubka 2, 3
Affiliation  

Bradymyces oncorhynchi is a poorly known melanised fungal species that has been isolated only from a hyperaemic focus near the enlarged spleen of a rainbow trout. Although the pathogenicity of this species to fish is suspected, it has not been fully confirmed. Four laboratory experiments were conducted to test the effect of the fungus on the health of rainbow trout fingerlings. Mycelia were cultivated under different conditions to increase inoculum variability and to test the impact of the conditions on the pathogenicity. The inoculum was subsequently administered by the intraperitoneal route. The clinical manifestations, gross pathological lesions and histopathological changes were identical in all experimental groups, i.e. lethargy, inappetence, anorexia, weight reduction, pale gills, full-thickness ulceration of the abdominal wall, muscle atrophy, haemorrhagic or pale liver, fat-altered pyloric region with haemorrhages, enlarged spleen, haemorrhagic ascites, systemic granulomatous lesions with the presence of melanised hyphae (phaeohyphomycosis), multifocal granulomatous hepatitis, perivascular lymphocytic infiltration, vacuolation of hepatocytes and vacuolation of the kidney tubule epithelium. No statistically significant differences were found in the survival of the fish with respect to experimental settings. The survival period ranged between 3 and 135 days.



中文翻译:

实验性感染真菌Bradymyces oncorhynchi的虹鳟Oncorhynchus mykiss的临床和组织病理学变化。

缓生猪笼草是一种鲜为人知的黑色素化真菌,仅从虹鳟扩大的脾脏附近的高氧病灶中分离出来。尽管怀疑该物种对鱼类的致病性,但尚未完全证实。进行了四个实验室实验,以测试真菌对虹鳟鱼幼鱼健康的影响。在不同条件下培养菌丝体,以增加接种物的变异性,并测试条件对致病性的影响。随后通过腹膜内途径施用接种物。所有实验组的临床表现,总体病理病变和组织病理学变化均相同,即嗜睡,食欲不振,厌食,体重减轻,腮,、腹壁全层溃疡,肌肉萎缩,出血性或苍白的肝,肥大的幽门区域伴有出血,脾脏肿大,出血性腹水,全身肉芽肿性病变并伴有黑色素化的菌丝(噬菌毛病),多灶性肉芽肿性肝炎,血管周淋巴细胞浸润,肝小管空泡化和肾小管上皮细胞空泡化。就实验设置而言,在鱼类的存活率上没有发现统计学上的显着差异。生存期为3至135天。就实验设置而言,在鱼类的存活率上没有发现统计学上的显着差异。生存期为3至135天。就实验设置而言,在鱼类的存活率上没有发现统计学上的显着差异。生存期为3至135天。

更新日期:2020-07-30
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