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A chondroprotective effect of moracin on IL-1β-induced primary rat chondrocytes and an osteoarthritis rat model through Nrf2/HO-1 and NF-κB axes.
Food & Function ( IF 6.1 ) Pub Date : 2020-07-29 , DOI: 10.1039/d0fo01496f
Siqi Zhou 1 , Jiaqi Shi 2 , Haiyan Wen 3 , Wei Xie 1 , Xiaotao Han 4 , Haohuan Li 1
Affiliation  

Osteoarthritis (OA) is a common joint disease characterized by cartilage degeneration and inflammation. Although moracin is known to play a role in anti-inflammation and anti-oxidation in several inflammatory diseases, its anti-inflammatory effect on OA remains largely unknown. Therefore, in order to explore the role of moracin in OA, we investigated the anti-inflammatory effect of moracin on interleukin (IL)-β-induced rat chondrocytes in vitro and surgically induced OA rat models in vivo. Rat chondrocytes were pretreated using moracin (0, 5, 10, 15 μmol L−1) and then stimulated with IL-β (10 ng ml−1). Results showed that moracin reduced the expression of IL-1β-induced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in both rat chondrocytes and cell culture supernatants. Besides, IL-1β-induced degradation of aggrecan and collage II, and the high expression of matrix metalloproteinase-13 (MMP-13) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS)-5 were also reversed by moracin. Moreover, moracin inhibited the translocation of p65 from the cytoplasm to nucleus induced by IL-1β and activated the Nrf2/HO-1 signaling pathway in chondrocytes. In OA rat models, moracin prevented cartilage of rats from destruction. All these findings above indicated that moracin could be a potentially effective drug for treating OA.

中文翻译:

苦橙素通过Nrf2 / HO-1和NF-κB轴对IL-1β诱导的原代大鼠软骨细胞和骨关节炎大鼠模型的软骨保护作用。

骨关节炎(OA)是一种常见的关节疾病,其特征在于软骨变性和炎症。尽管已知morracin在几种炎性疾病中可起到抗发炎和抗氧化的作用,但其对OA的抗炎作用仍然未知。因此,为了探讨OA moracin的作用,我们研究了在白细胞介素(IL)-β-诱导的大鼠软骨细胞moracin的抗炎作用的体外和手术诱导的OA模型大鼠体内。大鼠用软骨素(0、5、10、15μmolL -1)预处理软骨细胞,然后用IL-β(10 ng ml -1)刺激)。结果显示,morracin降低了IL-1β诱导的一氧化氮(NO),前列腺素E2(PGE2),肿瘤坏死因子-α(TNF-α),IL-6,诱导型一氧化氮合酶(iNOS)和环氧合酶的表达大鼠软骨细胞和细胞培养上清液中的(COX)-2。此外,草甘膦还可以逆转IL-1β诱导的聚集蛋白聚糖和胶原II的降解以及基质金属蛋白酶13(MMP-13)和具有血小板反应蛋白基序5(ADAMTS)-5的整合素和金属蛋白酶的高表达。此外,苦橙素抑制IL-1β诱导的p65从细胞质向核的转运,并激活软骨细胞中的Nrf2 / HO-1信号通路。在OA大鼠模型中,苦味素可防止大鼠软骨受到破坏。以上所有这些发现表明,草蛋白可能是治疗OA的潜在有效药物。
更新日期:2020-09-23
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