Pluripotent stem cells are promising candidates for cell-based regenerative therapies. To avoid rejection of transplanted cells, several approaches are being pursued to reduce immunogenicity of the cells or modulate the recipient's immune response. These include gene editing to reduce the antigenicity of cell products, immunosuppression of the host, or using major histocompatibility complex-matched cells from cell banks. In this context, we have investigated the antigenicity of H-Y antigens, a class of minor histocompatibility antigens encoded by the Y chromosome, to assess whether the gender of the donor affects the cell's antigenicity. In a murine transplant model, we show that the H-Y antigen in undifferentiated embryonic stem cells (ESCs), as well as ESC-derived endothelial cells, provokes T- and B cell responses in female recipients.

中文翻译：

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胚胎干细胞中的 HY 抗原导致同基因女性受体的排斥反应。

多能干细胞是基于细胞的再生疗法的有希望的候选者。为了避免对移植细胞的排斥，正在寻求几种方法来降低细胞的免疫原性或调节受体的免疫反应。这些包括基因编辑以降低细胞产物的抗原性、宿主的免疫抑制或使用来自细胞库的主要组织相容性复合体匹配的细胞。在此背景下，我们研究了 HY 抗原（一类由 Y 染色体编码的次要组织相容性抗原）的抗原性，以评估供体的性别是否影响细胞的抗原性。在小鼠移植模型中，我们显示未分化胚胎干细胞 (ESC) 以及 ESC 衍生的内皮细胞中的 HY 抗原会在女性受体中引发 T 细胞和 B 细胞反应。