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Molecular Characterization and Survival of Carbapenem-Resistant Acinetobacter baumannii Isolated from Hospitalized Patients in Mostar, Bosnia and Herzegovina
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2021-03-12 , DOI: 10.1089/mdr.2020.0163
Sanja Jakovac 1 , Ivana Goić-Barišić 2 , Mario Pirija 3 , Ana Kovačić 4 , Jasna Hrenović 5 , Tanja Petrović 1 , Borka Tutiš 6 , Marija Tonkić 2
Affiliation  

Increasingly difficult treatment of multidrug-resistant (MDR) bacteria has become a global problem of the 21st century. Within a group of multiresistant bacteria, the Acinetobacter baumannii convincingly occupies the position at the top of the group designated as ESKAPE pathogens. In this study, 61 isolates of A. baumannii were recovered from different samples originating from various departments of the University Clinical Hospital Mostar during 2018. All of the isolates were identified using conventional phenotypic methods and the VITEK® 2 Compact System, and were confirmed by MALDI-TOF mass spectrometry. The minimum inhibitory concentrations (MICs) were determined by the microbroth dilution method using MICRONAUT-S MDR MRGN-Screening and VITEK 2 Compact System. All strains were resistant to carbapenems and classified in eight different resistotypes according to their antibiotic resistance and macrorestriction pulsed-field gel electrophoresis profiles, with all belonging to IC II. One isolate displayed resistance to colistin (MIC ≥16 mg/L). The presence of blaOXA genes encoding OXA-type carbapenemases was investigated by multiplex PCR and the Eazyplex® SuperBugAcineto system and showed 100% compatibility with the detection of acquired oxacillinases. Molecular characterization of the isolates tested in this study revealed the OXA-23- and OXA-40-like groups of acquired oxacillinases. Sequencing of two PCR products of the OXA-40-like group confirmed the presence of OXA-72. Survival assays with two selected isolates of A. baumannii encoding different mechanisms of carbapenem resistance revealed that one isolate was able to survive on a fragment of white laboratory coat during 90 days of monitoring. To the best of our knowledge, this is the first article to present the results of a comprehensive phenotypic, genotypic, and molecular analysis of A. baumannii isolates from the leading clinical hospital center in the southwestern part of Bosnia and Herzegovina, including data for the survival of this pathogen on the white laboratory coats used as compulsory medical clothing.

中文翻译:

从波斯尼亚和黑塞哥维那莫斯塔尔住院患者中分离出的耐碳青霉烯鲍曼不动杆菌的分子特征和存活率

耐多药 (MDR) 细菌越来越难以治疗,已成为 21 世纪的全球性问题。在一组多重耐药细菌中,鲍曼不动杆菌令人信服地占据了被指定为 ESKAPE 病原体组的顶部位置。在这项研究中,61株鲍曼不动杆菌是在2018年全部采用传统的表型方法和VITEK鉴定的菌株来自各部门的大学临床医院莫斯塔尔的不同样品发起恢复®2 Compact System,并通过 MALDI-TOF 质谱法确认。最低抑菌浓度 (MIC) 通过微量肉汤稀释法使用 MICRONAUT-S MDR MRGN-Screening 和 VITEK 2 Compact System 确定。所有菌株均对碳青霉烯类耐药,并根据其抗生素耐药性和宏观限制脉冲场凝胶电泳谱分为八种不同的耐药型,均属于 IC II。一种分离株显示出对粘菌素的耐药性(MIC ≥16 mg/L)。通过多重 PCR 和 Eazyplex ®研究了编码 OXA 型碳青霉烯酶的bla OXA 基因的存在SuperBugAcineto 系统并显示出与获得性苯唑西林酶检测 100% 的兼容性。本研究中测试的分离物的分子特征揭示了获得性苯唑西林酶的 OXA-23 和 OXA-40 样组。OXA-40 样组的两个 PCR 产物的测序证实了 OXA-72 的存在。对编码不同碳青霉烯类耐药机制的两种鲍曼不动杆菌的选定分离株进行的存活分析表明,在 90 天的监测期间,一种分离株能够在白色实验室外套的碎片上存活。据我们所知,这是第一篇介绍鲍曼不动杆菌综合表型、基因型和分子分析结果的文章 来自波斯尼亚和黑塞哥维那西南部主要临床医院中心的分离物,包括该病原体在用作强制医疗服的白色实验室外套上的存活数据。
更新日期:2021-03-17
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