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High-throughput SARS-CoV-2 and host genome sequencing from single nasopharyngeal swabs.
medRxiv - Genetic and Genomic Medicine Pub Date : 2020-09-01 , DOI: 10.1101/2020.07.27.20163147
John E Gorzynski , Hannah N De Jong , David Amar , Chris R Hughes , Alexander Ioannidis , Rob Bierman , Darren Liu , Yosuke Tanigawa , Amy Kistler , Jack Kamm , Jaehee Kim , Lorenzo Cappello , Norma F Neff , Simone Rubinacci , Olivier Delaneau , Massa J Shoura , Kinya Seo , Anna Kirillova , Archana Raja , Shirley Sutton , ChunHong Huang , Malaya Kumar Sahoo , Kalyan C Mallempati , Gonzalo Montero-Martin , Kazutoyo Osoegawa , David Jimenez-Morales , Nathaniel Watson , Nathan Hammond , Ruchi Joshi , Marcelo Fernandez-Vina , Jeffrey W Christle , Matthew T Wheeler , Phil Febbo , Kyle Farh , Gary Schroth , F Desouza , Julia Palacios , Julia Salzman , Benjamin A Pinsky , Manuel A Rivas , Carlos D Bustamante , Euan A Ashley , Victoria N Parikh

During COVID19 and other viral pandemics, rapid generation of host and pathogen genomic data is critical to tracking infection and informing therapies. There is an urgent need for efficient approaches to this data generation at scale. We have developed a scalable, high throughput approach to generate high fidelity low pass whole genome and HLA sequencing, viral genomes, and representation of human transcriptome from single nasopharyngeal swabs of COVID19 patients.

中文翻译:

来自单个鼻咽拭子的高通量 SARS-CoV-2 和宿主基因组测序。

在 COVID19 和其他病毒大流行期间,快速生成宿主和病原体基因组数据对于跟踪感染和通知治疗至关重要。迫切需要有效的方法来大规模生成这种数据。我们开发了一种可扩展的高通量方法,以从 COVID19 患者的单个鼻咽拭子中生成高保真低通全基因组和 HLA 测序、病毒基因组和人类转录组的表征。
更新日期:2020-09-01
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