当前位置: X-MOL 学术ACS Chem. Neurosci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Myelination and Node of Ranvier Formation in a Human Motoneuron-Schwann Cell Serum-Free Coculture.
ACS Chemical Neuroscience ( IF 5 ) Pub Date : 2020-07-28 , DOI: 10.1021/acschemneuro.0c00287
Aakash Patel 1 , John W Rumsey 2 , Case Lorance 2 , Christopher J Long 2 , Briana Lee 1 , Laurene Tetard 1 , Stephen Lambert 1 , James J Hickman 1, 2
Affiliation  

Myelination and node of Ranvier formation play an important role in the rapid conduction of nerve impulses, referred to as saltatory conduction, along axons in the peripheral nervous system. We report a human–human myelination model using human primary Schwann cells (SCs) and human-induced pluripotent stem-cell-derived motoneurons utilizing a serum-free medium supplemented with ascorbate to induce myelination, where 41.6% of SCs expressed the master transcription factor for myelination, early growth response protein 2. After 30 days in coculture, myelin segments were visualized using immunocytochemistry for myelin basic protein surrounding neurofilament-stained motor neuron axons, which was confirmed via 3D confocal Raman microscopy, a viable alternative for transmission electron microscopy analysis. The myelination efficiency was 65%, and clusters of voltage-gated sodium channels and the paranodal protein contactin-associated protein 1 indicated node of Ranvier formation. This model has applications to study remyelination and demyelinating diseases, including Charcot–Marie Tooth disorder, Guillian–Barre syndrome, and anti-myelin-associated glycoprotein peripheral neuropathy.

中文翻译:

人Motoneuron-Schwann细胞无血清共培养物中的髓鞘形成和Ranvier形成的节点。

Ranvier形成的髓鞘形成和结节在沿周围神经系统的轴突的神经冲动的快速传导(称为盐分传导)中起重要作用。我们报道了使用人原发性施万细胞(SCs)和人诱导多能干细胞衍生的运动神经元的人-人髓鞘形成模型,利用无血清培养基补充抗坏血酸诱导髓鞘形成,其中41.6%的SC表示主转录因子共培养30天后,使用免疫细胞化学技术对神经丝染色的运动神经元轴突周围的髓鞘碱性蛋白进行免疫细胞化学可视化髓鞘节段,并通过3D共聚焦拉曼显微镜证实了这一点,这是透射电镜分析的一种可行替代方法。髓鞘形成效率为65%,电压门控性钠通道簇和旁结蛋白接触蛋白相关蛋白1指示Ranvier形成的节点。该模型可用于研究髓鞘再生和脱髓鞘疾病,包括Charcot–Marie牙病,Guillian–Barre综合征和抗髓磷脂相关糖蛋白周围神经病。
更新日期:2020-09-02
down
wechat
bug