Abstract

The porous polylactic acid matrix has been particularly developed as a scaffold in tissue engineering, drug loading, and wound dressing. However, the loading of active chemical compounds is challenging due to its highly hydrophobic nature and lack of functional groups for chemical bonding. Plasma treatment is a fast, heat and chemical-free solution to increase its hydrophilicity by oxidative functional groups and physical etching. In this study, a highly porous PLA scaffold was obtained by polyethylene glycol pore formation agent and treated by radiofrequency (RF) air plasma. Fucoidan from Fucus vesiculosus, Macrocystis pyrifera, and Undaria pinnatifida were used to immobilize onto the activated porous PLA surface for use as medicated wound dressings. The chemical composition and morphology were investigated by FTIR, SEM-EDS analysis, and cytocompatibility assay was studied mouse embryonic fibroblast cells (NIH/3T3).

摘要

多孔聚乳酸基质已被特别开发为组织工程、载药和伤口敷料中的支架。然而，由于其高度疏水性和缺乏用于化学键合的官能团，活性化合物的负载具有挑战性。等离子处理是一种快速、加热和无化学物质的解决方案，通过氧化官能团和物理蚀刻来增加其亲水性。在这项研究中，通过聚乙二醇造孔剂获得了高度多孔的 PLA 支架，并通过射频 (RF) 空气等离子体处理。来自Fucus vesiculosus、Macrocystis pyrifera和Undaria pinnatifida 的褐藻糖胶用于固定在活化的多孔 PLA 表面上，用作药物伤口敷料。通过FTIR、SEM-EDS分析和细胞相容性分析研究了小鼠胚胎成纤维细胞(NIH/3T3)的化学组成和形态。