Abstract

Chronic unpredictable mild stress (CUMS) is a valid model for inducing depression-like symptoms in animal models, causing predictive behavioral, neurochemical, and physiological responses to this condition. This work aims to evaluate the possible antidepressant effect of γ-oryzanol (ORY) in the CUMS-induced depressive model in male Drosophila melanogaster. We will use the CUMS protocol to continue the study previously conducted by our research group, mimicking a depressive state in these insects. Male flies were subjected to various stressors according to a 10-day randomized schedule and concomitantly treated with ORY or fluoxetine (FLX). After the experimental period, in vivo behavioral tests were performed (open field, forced swimming, aggressiveness test, mating test, male virility, sucrose preference index and light/dark test) and ex vivo analyses measuring serotonin (5HT), dopamine (DA), octopamine (OCT) levels and body weight. We report here that ORY-treated flies and concomitant exposure to CUMS did not exhibit obvious behaviors such as prolonged immobility or increased aggressive behavior, reduced male mating and virility behavior, and anxiolytic behavior, in contrast to ORY, not altering sucrose preference and body weight flies exposed to CUMS. ORY effectively prevented 5HT and OCT reduction and partially protected against DA reduction. The data presented here are consistent and provide evidence for the use of ORY as a potential antidepressant compound.

• Lay Summary

• Flies treated with ORY and concomitant exposure to CUMS did not exhibit obvious depressive-like behaviors, such as prolonged immobility in the FST or increased aggressive behavior, or reduced mating behavior, male virility, or anxiolytic behavior. ORY did not change the preference for sucrose and body weight of flies, about the levels of monoamines in the heads of flies, ORY was effective in preventing the reduction of 5HT and OCT, and we had partial protection of ORY for reducing the levels of DA.

摘要

慢性不可预测的轻度应激（CUMS）是一种有效的模型，可在动物模型中诱发抑郁症样症状，从而引起对此情况的预测性行为，神经化学和生理反应。这项工作旨在评估γ-谷维素（ORY）在CUMS诱导的男性果蝇抑郁模型中的抗抑郁作用。我们将使用CUMS方案继续我们的研究小组先前进行的研究，以模拟这些昆虫的抑郁状态。根据10天的随机时间表，对雄蝇进行各种应激处理，并同时进行ORY或氟西汀（FLX）处理。在实验期之后，进行了体内行为测试（开阔地域，强迫游泳，攻击性测试，交配测试，雄性，蔗糖偏爱指数和明/暗测试）和离体测试分析测量血清素（5HT），多巴胺（DA），章鱼胺（OCT）含量和体重。我们在这里报告说，与ORY相比，经ORY处理的果蝇并伴有CUMS暴露，并未表现出明显的行为，例如长时间的不动或增加的攻击行为，减少的男性交配和性行为以及抗焦虑行为，而不改变蔗糖的偏好和体重苍蝇暴露于CUMS。ORY有效地防止了5HT和OCT的降低，并部分保护了DA的降低。此处提供的数据是一致的，并为使用ORY作为潜在的抗抑郁化合物提供了证据。

• 放置摘要

• 用ORY处理并同时暴露于CUMS的果蝇没有表现出明显的抑郁样行为，例如FST长时间不动或侵略行为增加，或交配行为减少，雄性或抗焦虑行为。对于果蝇头部中的单胺水平，ORY不会改变对蔗糖和果蝇的偏爱，ORY可以有效防止5HT和OCT的降低，并且我们对ORY起到了部分保护作用，可以降低DA的水平。